| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,622,669 | T→C | 48.3% | A659A (GCA→GCG) | maeB ← | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,622,669 | 0 | T | C | 48.3% | ‑3.7 / 50.6 | 58 | A659A (GCA→GCG) | maeB | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base T (8/22); new base C (14/14); total (22/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAG > minE/1622604‑1622723 | tCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCTTGCATTTCACCATCAATCATCAGTTCTGGTGct < 1:765807/69‑2 (MQ=255)tCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCa < 1:3315921/69‑1 (MQ=255)tCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCa < 1:2637811/69‑1 (MQ=255)tCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCa < 1:2289683/69‑1 (MQ=255)tCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCa < 1:2095085/69‑1 (MQ=255)tCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCa < 1:1568488/69‑1 (MQ=255) cGTTGCGAAGCGCTTCCACCAGCGCTGCATCTCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCa < 1:1689988/68‑1 (MQ=255) gattCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:1990363/65‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:1046667/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:876570/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:661809/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:642557/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:377405/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:346821/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:2559381/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:1773804/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:1161134/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:1303701/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:1578146/68‑1 (MQ=255) gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:1408644/68‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCCTCAATCATCAGTTCTGGTGCACGTTCCCt < 1:3110789/69‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:1747056/69‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:476329/69‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:472298/69‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:1368314/69‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:3312059/69‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:2934315/69‑1 (MQ=255) aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:2699653/69‑1 (MQ=255) cgcTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGCGCACGTTcc < 1:247934/52‑1 (MQ=255) cGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCa < 1:1721568/69‑1 (MQ=255) cGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCa < 1:535483/69‑1 (MQ=255) cGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCa < 1:508861/69‑1 (MQ=255) cGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCa < 1:2684222/69‑1 (MQ=255) aTTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCt < 1:2798192/38‑1 (MQ=255) tCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcgac > 1:1417259/1‑69 (MQ=255) tCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcgac > 1:888139/1‑69 (MQ=255) tCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcgac > 1:1044296/1‑69 (MQ=255) tCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcgac > 1:654242/1‑69 (MQ=255) tCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcgac > 1:1378898/1‑69 (MQ=255) tCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcgac > 1:3125163/1‑69 (MQ=255) tCACAATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGc > 1:908545/1‑57 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGa > 1:2818083/1‑50 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:1525657/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:2890939/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:1681721/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:1599035/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:1463517/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:1439118/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:234532/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:2363110/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:3237907/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:3207708/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTc > 1:3138881/1‑62 (MQ=255) cATCAATCATCAGTTCTGGCGCACGTTCCCTGACCAGTTACAGCGCCTGACGCATTTTGCTc > 1:1777109/1‑62 (MQ=255) cATCAAACATCAGTTCTGGCGCACGTTCCCTGAGCAGTTCCAGCGCCTGACGCATTTTGCt > 1:196340/1‑61 (MQ=255) tcatcaGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAg < 1:2567502/68‑1 (MQ=255) catcaGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcgac > 1:1156044/1‑58 (MQ=255) catcaGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAg < 1:2343724/67‑1 (MQ=255) | TCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAG > minE/1622604‑1622723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |