| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,622,786 | G→A | 23.2% | V620V (GTC→GTT) | maeB ← | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,622,786 | 0 | G | A | 23.2% | 120.4 / 27.7 | 90 | V620V (GTC→GTT) | maeB | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base G (26/43); new base A (14/7); total (40/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
CAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGT > minE/1622721‑1622854 | cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:1047569/69‑1 (MQ=255)cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:1586941/69‑1 (MQ=255)cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:677762/69‑1 (MQ=255)cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:2575243/69‑1 (MQ=255)cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:1340626/69‑1 (MQ=255)cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:2055830/69‑1 (MQ=255)cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:283970/69‑1 (MQ=255)cAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:3307588/69‑1 (MQ=255) aGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACa < 1:1028001/68‑1 (MQ=255) aagaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAATCGACGGACAGTTTCTg > 1:1416831/1‑69 (MQ=255) aagaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCt > 1:3346797/1‑68 (MQ=255) aagaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCt > 1:1237461/1‑68 (MQ=255) aagaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCt > 1:913946/1‑68 (MQ=255) aagaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTg > 1:496521/1‑69 (MQ=255) aagaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTg > 1:3264058/1‑69 (MQ=255) aagaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTg > 1:2062090/1‑69 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTc > 1:2371016/1‑66 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1912248/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1710183/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:2108726/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1755462/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1966508/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1476204/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1369787/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:2794306/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1338927/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1234684/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:1210821/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:582224/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAGAGCCACACGCGGCTCAATACCAAAACGACGAACAGTTTCt > 1:712193/1‑67 (MQ=255) agaaCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTg > 1:250222/1‑68 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:2688252/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:2446741/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:2442884/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:2806175/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:133346/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:2552385/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:1965640/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:3057996/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:2114340/69‑1 (MQ=255) aCCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:72926/69‑1 (MQ=255) cAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGAGGGACa < 1:2465229/56‑1 (MQ=255) ggAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAATGACGGa < 1:436739/47‑1 (MQ=255) ggAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGa < 1:1588996/47‑1 (MQ=255) ggAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGa < 1:529473/47‑1 (MQ=255) ggAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGa < 1:876986/47‑1 (MQ=255) ggAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGa < 1:2500164/47‑1 (MQ=255) ggAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGa < 1:1344125/47‑1 (MQ=255) ggAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGa < 1:2756628/47‑1 (MQ=255) gAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGa < 1:804240/46‑1 (MQ=255) aaGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGct > 1:2906951/1‑69 (MQ=255) aaGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGc > 1:981131/1‑68 (MQ=255) aaGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATAAAGGTGATCTCCGCCAGct > 1:2951846/1‑69 (MQ=255) aGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgc < 1:2866256/45‑1 (MQ=255) cacgcgGCTCAATACCAAAACGACGAACAGTTTCTGCCGCCATCAAGGTGAtct < 1:3358009/52‑1 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:2693684/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1349014/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1066055/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:739402/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1140680/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:2816531/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:2839139/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1481388/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1149543/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:3389829/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:2225783/1‑68 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:1814415/1‑67 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:1621865/1‑67 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:2503416/1‑67 (MQ=255) ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:2349717/1‑67 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:899314/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:882526/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:2011391/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:1318082/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:2039153/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:2396684/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:2706509/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:980800/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:871230/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:865923/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:1019936/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:1624093/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:287371/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:1283077/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:2173916/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:3025529/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:3317568/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:1327998/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:3302927/69‑1 (MQ=255) acgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACata < 1:602429/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:439527/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:1124038/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:391823/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:391619/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:2246157/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:3385806/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:3337888/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:1333329/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:3181425/69‑1 (MQ=255) | CAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGT > minE/1622721‑1622854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |