| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,622,837 | T→C | 35.6% | E603E (GAA→GAG) | maeB ← | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,622,837 | 0 | T | C | 35.6% | 81.1 / 79.6 | 129 | E603E (GAA→GAG) | maeB | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base T (30/53); new base C (40/6); total (70/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-02 | |||||||||||
Only 100 of 129 total aligned reads displayed.
CCAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGTTCATGGC > minE/1622775‑1622902 | ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1066055/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1481388/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:2225783/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1349014/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:2816531/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:2839139/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:3389829/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:739402/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcatc > 1:1140680/1‑68 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:1621865/1‑67 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:1814415/1‑67 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:2349717/1‑67 (MQ=255)ccAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTtcat > 1:2503416/1‑67 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:2396684/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:2011391/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:2039153/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:882526/69‑1 (MQ=255) aaaaCGACGAACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCAGGCTCATCAtt < 1:1318082/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:2706509/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:871230/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:1019936/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:2173916/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:287371/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:980800/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:3025529/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:3302927/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:3317568/69‑1 (MQ=255) cgacgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:1327998/69‑1 (MQ=255) acgGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACata < 1:602429/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:1124038/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:1333329/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:3181425/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:3385806/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:391619/69‑1 (MQ=255) gACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:439527/69‑1 (MQ=255) aCAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:2280226/68‑1 (MQ=255) tCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGc > 1:115445/1‑68 (MQ=255) tCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGc > 1:1114487/1‑68 (MQ=255) tccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:790176/67‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:591290/68‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:287652/68‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:1084464/68‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:2828949/68‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:2490570/68‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:2470840/68‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:978578/68‑1 (MQ=255) gccATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:1721937/68‑1 (MQ=255) gacATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATaaa < 1:2730452/66‑1 (MQ=255) aaGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACa < 1:1349927/45‑1 (MQ=255) aaGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGtt > 1:1708511/1‑68 (MQ=255) aaGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTa > 1:2512554/1‑69 (MQ=255) tcCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGt < 1:1394466/41‑1 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgctac > 1:1588938/1‑66 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:3076881/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:3104571/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:1176476/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:3362321/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:3329914/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:1272121/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:1889509/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:1153807/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:1098911/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:2034656/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:759708/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:766845/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:2145147/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:879063/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:247770/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:225068/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:2281199/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:2383159/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgcaac > 1:236669/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgca > 1:144459/1‑67 (MQ=255) gCCAGCTCTTCTGCATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGgc > 1:1173903/1‑66 (MQ=255) ctGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGcgcg > 1:471572/1‑68 (MQ=255) ctGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGcgcg > 1:3161774/1‑68 (MQ=255) ctGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGcgcg > 1:314644/1‑68 (MQ=255) ctGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGcgcg > 1:2988808/1‑68 (MQ=255) ctGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGcgcg > 1:960554/1‑68 (MQ=255) ctGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGcgcg > 1:771615/1‑68 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATGAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:1421549/68‑1 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:1255160/68‑1 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:570666/68‑1 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:1868568/68‑1 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:1517257/68‑1 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:2189861/68‑1 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:2716995/68‑1 (MQ=255) gCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGtt < 1:2576106/68‑1 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTc > 1:2961017/1‑68 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:1641911/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:449800/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:1906935/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:806027/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:263565/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:1265427/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:654662/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:624280/1‑69 (MQ=255) cATCAGGCTCATCATTGACGTAGGTATCGGCAATAAAGGTGTTACCACTCGGCAACAGCAGCGCGTTCa > 1:1614387/1‑69 (MQ=255) cGGTTCATCATTAACATATGTATCGGCAATAAAGgtgt > 1:2807273/1‑38 (MQ=255) cGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGTTCATGGc < 1:1284407/69‑1 (MQ=255) | CCAAAACGACGGACAGTTTCTGCCGCCATCAAGGTGATCTCCGCCAGCTCTTCTGCATCCGGTTCATCATTAACATATGTATCGGCAATAAAGGTGTTACCACTCGGCAGCAGCAGCGCGTTCATGGC > minE/1622775‑1622902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |