BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,625,686	G→A	48.9%	L251L (CTG→CTA)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,625,686	0	G	A	48.9%	‑2.2 / 37.6	45	L251L (CTG→CTA)	talA	transaldolase A
Reads supporting (aligned to +/- strand): ref base G (7/16); new base A (13/9); total (20/25)
Fisher's exact test for biased strand distribution p-value = 7.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCT  >  minE/1625624‑1625731

cAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGa                                          >  1:893354/1‑68 (MQ=255)

      cTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGt                                    <  1:2171118/68‑1 (MQ=255)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:1084521/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:80998/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:696296/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:3311216/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:3252421/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:2298231/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:1452148/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:1165466/69‑1 (MQ=38)

           cTTAACCGGCTGCGACCGACTGACTATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGcc                              <  1:1267816/69‑1 (MQ=38)

                    cTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACg                     <  1:1257599/69‑1 (MQ=255)

                    cTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACg                     <  1:2200530/69‑1 (MQ=255)

                     tGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACg                     <  1:3101339/68‑1 (MQ=255)

                          tCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAAtt               <  1:1068485/69‑1 (MQ=255)

                          tCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAAtt               <  1:3356215/69‑1 (MQ=255)

                           cGACTTACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              <  1:127947/69‑1 (MQ=255)

                           cGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              >  1:1114997/1‑69 (MQ=255)

                           cGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              <  1:3301760/69‑1 (MQ=255)

                           cGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              <  1:1208348/69‑1 (MQ=255)

                           cGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              <  1:369828/69‑1 (MQ=255)

                           cGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              <  1:557080/69‑1 (MQ=255)

                            gactgactATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              <  1:2740843/68‑1 (MQ=255)

                            gactgactATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTa              <  1:2864003/68‑1 (MQ=255)

                                gactATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATccc         <  1:2321562/69‑1 (MQ=255)

                                gactATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATccc         <  1:3236972/69‑1 (MQ=255)

                                gactATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATccc         <  1:3240913/69‑1 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:47712/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:643989/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:1929701/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:3311762/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:167965/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:3307421/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:2222553/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:3129412/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:2121087/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:2670825/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:25490/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:225377/1‑69 (MQ=255)

                                  ctATCGCACCGAATTTGCTGAAGGAGCTACAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCAc       >  1:2194285/1‑69 (MQ=255)

                                       gCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCtt    >  1:2022624/1‑67 (MQ=255)

                                       gCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCtt    >  1:2439038/1‑67 (MQ=255)

                                       gCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCtt    >  1:2415937/1‑67 (MQ=255)

                                       gCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCtt    >  1:1428539/1‑67 (MQ=255)

                                         aCCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTtct  >  1:1271889/1‑67 (MQ=255)

CAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCT  >  minE/1625624‑1625731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`