| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,625,936 | C→T | 39.3% | R11R (CGC→CGT) | tktB → | transketolase 2, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,625,936 | 0 | C | T | 39.3% | 66.0 / 81.8 | 122 | R11R (CGC→CGT) | tktB | transketolase 2, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (31/43); new base T (31/17); total (62/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CCAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATG > minE/1625874‑1625999 | ccAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTc < 1:1393633/69‑1 (MQ=255) aaaCTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCat < 1:2829974/69‑2 (MQ=255) aaaCTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAg < 1:2013469/69‑1 (MQ=255) aaCTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAg < 1:3018335/68‑1 (MQ=255) aaCTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAg < 1:51050/68‑1 (MQ=255) tataAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:2183184/69‑1 (MQ=255) tataAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:2064343/69‑1 (MQ=255) tataAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:1324657/69‑1 (MQ=255) tataAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:1697057/69‑1 (MQ=255) gAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACaaaaa > 1:2215444/1‑69 (MQ=255) gAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACaaaaa > 1:368771/1‑69 (MQ=255) gAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACaaaaa > 1:1339366/1‑69 (MQ=255) gAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACaaaaa > 1:3284820/1‑69 (MQ=255) gAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACaaaaa > 1:68100/1‑69 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTAt > 1:715407/1‑48 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:3020855/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg > 1:3147803/1‑49 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:3234606/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:119388/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:30349/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:2577549/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:3369913/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:1723624/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:1475004/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:2712514/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:1243200/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:2637800/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:130713/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:1358656/49‑1 (MQ=255) ttATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATg < 1:906853/49‑1 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAg < 1:1215732/40‑1 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAg < 1:3263383/40‑1 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAg < 1:2532102/40‑1 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAg < 1:258615/40‑1 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAActct > 1:488779/1‑68 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAActct > 1:3012838/1‑68 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:2417850/1‑69 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:2273669/1‑69 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:1943670/1‑69 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:2140491/1‑69 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:373367/1‑69 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:2805877/1‑69 (MQ=255) tGTCCCGAAAAGACCTTGCCAATGAGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:1023709/1‑69 (MQ=255) tCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGt > 1:2040637/1‑69 (MQ=255) tCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGt > 1:2397519/1‑69 (MQ=255) tCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGt > 1:681678/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:2479909/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:2726542/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:2061418/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:296884/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:3048464/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:3278930/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:1277167/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:1229248/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:1104030/1‑69 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:1734604/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:1459215/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:778749/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:2861694/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:1093132/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:954214/68‑1 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGTATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:648555/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:3296673/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2464373/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:539836/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:734957/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2634592/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1087918/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1415511/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1416057/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1919644/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1982947/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2412863/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2504303/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2621372/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2550767/1‑69 (MQ=255) aaGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATccc > 1:228045/1‑68 (MQ=255) aaGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCg > 1:2820480/1‑69 (MQ=255) tGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGcc < 1:415433/69‑1 (MQ=255) tGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGcc < 1:1954964/69‑1 (MQ=255) tGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGcc < 1:2089424/69‑1 (MQ=255) ccAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGa > 1:2024366/1‑69 (MQ=255) ccAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGa > 1:2519105/1‑69 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2192206/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:3056410/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:942172/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:913926/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:111743/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:3159474/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:1768007/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:1835294/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2410662/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:226248/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2454749/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:394137/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2239912/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2221211/63‑1 (MQ=255) gCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:1660726/62‑1 (MQ=255) aTTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATg < 1:1852300/69‑1 (MQ=255) aTTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATg < 1:2592237/69‑1 (MQ=255) | CCAAACTATAAACCAGCCACGGAGTGTTATATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATG > minE/1625874‑1625999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |