| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,625,969 | C→T | 39.3% | N22N (AAC→AAT) | tktB → | transketolase 2, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,625,969 | 0 | C | T | 39.3% | 80.9 / 102.6 | 150 | N22N (AAC→AAT) | tktB | transketolase 2, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (50/41); new base T (41/18); total (91/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 152 total aligned reads displayed.
TGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTA > minE/1625905‑1626036 | tGTCCCTAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:1366176/1‑69 (MQ=255)tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAActct > 1:488779/1‑68 (MQ=255)tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:2417850/1‑69 (MQ=255)tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:2273669/1‑69 (MQ=255)tGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTg > 1:1943670/1‑69 (MQ=255) tCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGt > 1:2040637/1‑69 (MQ=255) tCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGt > 1:681678/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:2631917/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:2534789/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:296884/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:3048464/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:3278930/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:2061418/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:1951425/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:1104030/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:1229248/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:95675/1‑69 (MQ=255) ccGAAAAGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCa > 1:1277167/1‑69 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:1459215/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:2804693/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:1734604/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:778749/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:2861694/68‑1 (MQ=255) gAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCAt < 1:954214/68‑1 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGTATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:648555/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1415511/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1982947/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:3296673/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:3046304/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1087918/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:734957/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2464373/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2504303/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:2634592/1‑69 (MQ=255) aaaaGACCTTGCCAATGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATcc > 1:1416057/1‑69 (MQ=255) aaGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCg > 1:1132382/1‑69 (MQ=255) tGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGcc < 1:2089424/69‑1 (MQ=255) tGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGcc < 1:415433/69‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2192206/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2224178/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:3159474/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:226248/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:111743/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:913926/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:671899/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:2410662/63‑1 (MQ=255) tGCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:1835294/63‑1 (MQ=255) gCGATTCGTGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGcc < 1:1660726/62‑1 (MQ=255) gCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGc > 1:2403086/1‑69 (MQ=255) gCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGc > 1:130006/1‑69 (MQ=255) aTTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATg < 1:2592237/69‑1 (MQ=255) aTTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATg < 1:1852300/69‑1 (MQ=255) gcACTCAGTATTGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:454252/68‑1 (MQ=255) gcACTCAGTATGTATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:1506437/68‑1 (MQ=255) gcACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:3199183/68‑1 (MQ=255) gcACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:2757015/68‑1 (MQ=255) gcACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:657303/68‑1 (MQ=255) aCTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:991150/66‑1 (MQ=255) cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa > 1:1020777/1‑63 (MQ=255) cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa > 1:317903/1‑63 (MQ=255) cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa > 1:3086112/1‑63 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTg > 1:2713838/1‑67 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:2453288/1‑68 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:383647/1‑68 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:2238516/1‑68 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:429270/1‑68 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:1587062/1‑68 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:2812059/1‑68 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:1182585/1‑68 (MQ=255) aGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGc > 1:2738851/1‑68 (MQ=255) tATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGa > 1:2464715/1‑69 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGa > 1:2350139/1‑45 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:2536156/58‑1 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:1598875/58‑1 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:1457932/58‑1 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa > 1:386371/1‑58 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:930509/58‑1 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:3347919/58‑1 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa > 1:3139434/1‑58 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:2796950/58‑1 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:281787/58‑1 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAg > 1:2609407/1‑69 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAg > 1:2179990/1‑69 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAg > 1:2343484/1‑69 (MQ=255) tGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAg > 1:479426/1‑69 (MQ=255) ggATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:409518/57‑1 (MQ=255) gATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGTCATGGCTGATATTGCCGAAGTg > 1:100983/1‑69 (MQ=255) gATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTg > 1:2423853/1‑69 (MQ=255) gATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTg > 1:266368/1‑69 (MQ=255) gATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTg > 1:1554936/1‑69 (MQ=255) gATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTg > 1:35794/1‑69 (MQ=255) tGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:1765636/54‑1 (MQ=255) tGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:1814134/54‑1 (MQ=255) tGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:1284804/54‑1 (MQ=255) tGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:1231671/54‑1 (MQ=255) tGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:190800/54‑1 (MQ=255) tGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGa < 1:209241/54‑1 (MQ=255) aaaaGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGACGAAGTGCTGTGGAACGa > 1:1041008/1‑69 (MQ=255) aCTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTa < 1:1975165/69‑1 (MQ=255) aCTCTGGTCATCCCGGCGCACCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTa < 1:2479947/69‑1 (MQ=255) | TGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTA > minE/1625905‑1626036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |