| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,626,713 | G→T | 21.9% | A270A (GCG→GCT) | tktB → | transketolase 2, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,626,713 | 0 | G | T | 21.9% | 88.6 / 15.6 | 50 | A270A (GCG→GCT) | tktB | transketolase 2, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base G (15/24); new base T (6/5); total (21/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
TAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTAT > minE/1626659‑1626773 | tAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCaaaa < 1:2169427/69‑1 (MQ=255) aaaGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCaaaa < 1:3283845/68‑1 (MQ=255) gTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCaaaa < 1:1587236/61‑1 (MQ=255) aaGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTgg > 1:1971983/1‑69 (MQ=255) aaGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCAcc > 1:2743024/1‑69 (MQ=255) aaGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCAcc > 1:610834/1‑69 (MQ=255) ggcgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATcc < 1:1533961/69‑1 (MQ=255) ggcgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATcc < 1:1017237/69‑1 (MQ=255) ggcgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATcc < 1:2907711/69‑1 (MQ=255) gcgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATcc < 1:490712/68‑1 (MQ=255) cgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGTCAAAAACTGGGCTGGCACCATCCGc > 1:2211721/1‑69 (MQ=255) cgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATCCGc > 1:2763271/1‑69 (MQ=255) cgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATCCGc > 1:128389/1‑69 (MQ=255) cgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATCCGc > 1:953960/1‑69 (MQ=255) cgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATCCGc > 1:2071657/1‑69 (MQ=255) cgcacggcgcacCGCTGGGGGAAGAAGAAGTAGCTCTGGCACGGCAAAAACTGGGCTGGCA‑CATCCGcc > 1:2244168/1‑69 (MQ=255) ggcgcacGACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGAACCATCCGCCAttt < 1:1644021/69‑1 (MQ=255) ggcgcacCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCAttt < 1:277024/69‑1 (MQ=255) caccacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:1605968/69‑1 (MQ=255) caccacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:2587654/69‑1 (MQ=255) caccacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:2830915/69‑1 (MQ=255) caccacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:22755/69‑1 (MQ=255) caccacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:2073839/69‑1 (MQ=255) caccacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:1425354/69‑1 (MQ=255) caccacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:718391/69‑1 (MQ=255) accacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:2386200/68‑1 (MQ=255) accacTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga < 1:1716010/68‑1 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGTCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATccc > 1:1675869/1‑66 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTgg > 1:1238034/1‑43 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTgaga > 1:2577763/1‑62 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATccc > 1:369657/1‑66 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATccc > 1:631521/1‑66 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATccc > 1:2681240/1‑66 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATccc > 1:3140632/1‑66 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATccc > 1:2455465/1‑66 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATccc > 1:2540871/1‑66 (MQ=255) gggggAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATcc > 1:3044081/1‑65 (MQ=255) aagaagaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAgaga < 1:2204240/69‑1 (MQ=255) aagaagaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAgaga < 1:2805580/69‑1 (MQ=255) aagaagaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAgaga < 1:1754023/69‑1 (MQ=255) gaagaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGAtt > 1:1628338/1‑69 (MQ=255) gaagaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGAtt > 1:93807/1‑69 (MQ=255) gaagaagTAGCTCTGGCACGGCAAAAACTGGGCTGGCACCATcc < 1:166314/44‑1 (MQ=38) agaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTa < 1:368652/69‑1 (MQ=255) agaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTa < 1:911332/69‑1 (MQ=255) agaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTa < 1:1268466/69‑1 (MQ=255) agaagTGGCGCTGGCACGGCAAAAACTGGGCTAGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTa < 1:201284/69‑1 (MQ=255) gaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTAt < 1:645566/69‑1 (MQ=255) gaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTAt < 1:682666/69‑1 (MQ=255) gaagTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTAt < 1:941856/69‑1 (MQ=255) | TAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTAT > minE/1626659‑1626773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |