| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 128,158 | C→T | 20.1% | L214L (CTG→TTG) | yadG → | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 128,158 | 0 | C | T | 20.1% | 117.1 / 18.1 | 79 | L214L (CTG→TTG) | yadG | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (47/16); new base T (2/14); total (49/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.29e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
CACACACTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTC > minE/128094‑128218 | cacacaCTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCtgg > 1:2398591/1‑68 (MQ=255)cacacaCTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCtgg > 1:1783143/1‑68 (MQ=255)cacacaCTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCtgg > 1:3056133/1‑68 (MQ=255)cacacaCTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCtgg > 1:515592/1‑68 (MQ=255) cTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGaaaa < 1:975273/69‑1 (MQ=255) cTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGaaaa < 1:231172/69‑1 (MQ=255) cTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGaaaa < 1:186050/69‑1 (MQ=255) cTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGaaaa < 1:759368/69‑1 (MQ=255) tACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGaaaa < 1:3100405/68‑1 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGaaa > 1:732442/1‑62 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:414436/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:1885498/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:1828545/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:221412/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:3055235/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:2088082/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:2714142/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTc > 1:414752/1‑69 (MQ=255) ggAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAACACCTc > 1:1736099/1‑69 (MQ=255) gaagCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTTGTGGAAAATACCTCGa > 1:2077246/1‑67 (MQ=255) gaagCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGa > 1:1649947/1‑67 (MQ=255) gaagCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGa > 1:318255/1‑67 (MQ=255) gaagCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGa > 1:142846/1‑67 (MQ=255) gaagCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGa > 1:406159/1‑67 (MQ=255) gaagCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGa > 1:2059054/1‑67 (MQ=255) gaagCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTAGa > 1:2909526/1‑67 (MQ=255) tgCCGCAATATCGGCATTATTCAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTgg > 1:125587/1‑67 (MQ=255) tgCCGCAATATCGGCATTATTCAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTgg > 1:2122301/1‑67 (MQ=255) ccGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGaa > 1:1913327/1‑69 (MQ=255) ccGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGa > 1:2251460/1‑68 (MQ=255) tCGTCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:2759122/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:1045330/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:869875/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:2760059/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:3139058/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:3246839/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:2318210/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:2060638/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:1070861/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:1052498/51‑1 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAAt > 1:764377/1‑69 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAAt > 1:728674/1‑69 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAAt > 1:1364471/1‑69 (MQ=255) tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAAt > 1:2844235/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGgcgc < 1:1734689/43‑1 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:699926/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:489398/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:2324854/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:1420860/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:3990/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:3430169/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:3323880/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:3296019/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:3287747/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:1544280/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:293752/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:2920734/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:1807008/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:2468273/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:2487213/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAcc > 1:1620346/1‑69 (MQ=255) attCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAAc > 1:2807377/1‑68 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:2098946/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:101725/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:941407/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:846961/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:2410885/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:1105935/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:1261121/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:2747614/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:1404722/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:1756806/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:3401598/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:1709034/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:1702309/68‑1 (MQ=255) cAACACGGTGAGTTGGTGGAAAATACTTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCAGAAACCtt < 1:1597458/68‑1 (MQ=255) acGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTAtt > 1:2464895/1‑68 (MQ=255) acGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATtc > 1:2176725/1‑69 (MQ=255) acGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATtc > 1:906238/1‑69 (MQ=255) | CACACACTACCTGGAAGAAGCAGAAATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTC > minE/128094‑128218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |