BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,628,398	G→A	63.0%	C216C (TGC→TGT)	ypfG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,628,398	0	G	A	63.0%	61.6 / 69.6	108	C216C (TGC→TGT)	ypfG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (14/26); new base A (28/40); total (42/66)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 109 total aligned reads displayed.

CAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAG  >  minE/1628338‑1628461

caGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAg                                                         >  1:956431/1‑69 (MQ=255)

caGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAg                                                         >  1:2270898/1‑69 (MQ=255)

                   agCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGag                                                                 <  1:3088599/42‑1 (MQ=255)

                    gcgcAGTGACATTCACATCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTc                                     >  1:391050/1‑69 (MQ=255)

                           gACATTCACCTCATGACGCAATGGATCAAGCGAGCAGCGCAGACCa                                                     >  1:2944150/1‑46 (MQ=255)

                            aCATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATc                              >  1:2888668/1‑68 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:351279/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:1442692/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:1023689/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:1681432/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:3137395/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:1362702/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:3343613/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:2096289/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:377655/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:819451/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:425384/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:486458/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:2215407/65‑1 (MQ=255)

                              aTTCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:782431/65‑1 (MQ=255)

                               ttCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:2162789/64‑1 (MQ=255)

                                tCACCTCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:2414318/63‑1 (MQ=255)

                                 cACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCaa                        >  1:2244380/1‑69 (MQ=255)

                                 cACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCaa                        >  1:393782/1‑69 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1015971/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:304776/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:81655/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2179851/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:604797/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:226834/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2312823/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2527519/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2667735/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2807311/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2808435/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2858975/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:3096793/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:3068855/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:2061405/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1248681/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1727356/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1301207/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1636845/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1164926/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:154363/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1069727/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1517481/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1358577/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1944028/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAAt                               >  1:1010909/1‑58 (MQ=255)

                                     tCACGACGTAACGGATCAAGCGAACAGCGCAGACCATTCATCCGACAGTTTCCATAAt                               >  1:1171009/1‑58 (MQ=255)

                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCg                    >  1:2344313/1‑68 (MQ=255)

                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCg                    >  1:248232/1‑68 (MQ=255)

                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCg                    >  1:190348/1‑68 (MQ=255)

                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGt                   >  1:3149325/1‑69 (MQ=255)

                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGt                   >  1:589045/1‑69 (MQ=255)

                                      cACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGt                   >  1:1023876/1‑69 (MQ=255)

                                                 ggATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCg        <  1:2784436/69‑1 (MQ=255)

                                                 ggATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCg        <  1:3434888/69‑1 (MQ=255)

                                                 ggATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCg        <  1:1109199/69‑1 (MQ=255)

                                                 ggATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCg        <  1:886923/69‑1 (MQ=255)

                                                 ggATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCg        <  1:3235193/69‑1 (MQ=255)

                                                    tCAAGCGAACAGCTCAGACCATTCATCCGCCAGTTTCCATAAt                               <  1:2610627/43‑1 (MQ=38)

                                                       aGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCg        <  1:463595/63‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCTAGTgag  <  1:2949765/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:1232397/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:576180/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:1343901/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:3188038/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:3112556/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:2177460/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:1993595/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:1863088/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:184879/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:1847655/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:2603873/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:177763/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:2890152/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:1418426/68‑1 (MQ=255)

                                                        gCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTgag  <  1:1429578/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:2535259/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:979250/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:948951/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:2252520/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:882570/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:1864037/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:3162637/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:2485408/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:1459306/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:568328/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:5554/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:50207/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:331003/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:2645303/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:1198413/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:1837206/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:3321427/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:282834/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGCGTgag  <  1:168194/68‑1 (MQ=255)

                                                        gCGAACAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTACGTTCTTCGAGTgag  <  1:1384840/68‑1 (MQ=255)

CAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAG  >  minE/1628338‑1628461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`