| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,629,654 | G→A | 37.8% | D31D (GAC→GAT) | yffH ← | predicted NUDIX hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,629,654 | 0 | G | A | 37.8% | 34.6 / 41.9 | 66 | D31D (GAC→GAT) | yffH | predicted NUDIX hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (30/11); new base A (8/17); total (38/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
AGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCTTT > minE/1629592‑1629722 | aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCttt > 1:838985/1‑68 (MQ=255)aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCTTTg > 1:1055048/1‑69 (MQ=255)aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCTTTg > 1:1211259/1‑69 (MQ=255)aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCTTTg > 1:595694/1‑69 (MQ=255)aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCTTTg > 1:306405/1‑69 (MQ=255)aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCTTTg > 1:2861500/1‑69 (MQ=255)aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCTTTg > 1:237746/1‑69 (MQ=255)aggaggATCGTCGCACCATTACCGCGATCGTAAACTTCACGTTTATGGCGGATAACTTCGCCATCTTTg > 1:2023582/1‑69 (MQ=255) ccATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCgta > 1:2722622/1‑68 (MQ=255) tGCCGCGATCGTACACTTGACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGt < 1:38613/55‑1 (MQ=255) tGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGt < 1:1186388/55‑1 (MQ=255) tGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGt < 1:764681/55‑1 (MQ=255) tGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGt < 1:3107985/55‑1 (MQ=255) tGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGt < 1:2576370/55‑1 (MQ=255) tGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGt < 1:195090/55‑1 (MQ=255) tGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGt < 1:1456822/55‑1 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:533264/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:3095670/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:3124948/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:2434388/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:77490/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:784841/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:837733/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:1783821/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:844593/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:1202021/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:1795857/1‑69 (MQ=255) gCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAAt > 1:2098592/1‑69 (MQ=255) aTCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTtgtg < 1:3028407/69‑1 (MQ=255) aTCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTtgtg < 1:2419507/69‑1 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:2524592/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:883082/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa < 1:1820057/69‑1 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:2889211/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:2815092/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:1693794/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:2493097/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:752173/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:2298566/1‑69 (MQ=255) cGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCa > 1:789382/1‑69 (MQ=255) tCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGaaa < 1:145474/69‑1 (MQ=255) tCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGa > 1:522723/1‑67 (MQ=255) tCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGa > 1:3334516/1‑67 (MQ=255) tCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGa > 1:3024440/1‑67 (MQ=255) tCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGa > 1:678055/1‑67 (MQ=255) tCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGa > 1:1725797/1‑67 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCTTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:735871/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:1332160/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:1833273/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:1859765/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:1962132/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:2264912/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:3005378/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:2427575/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:1607231/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:427527/69‑1 (MQ=255) gCGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:2925908/69‑1 (MQ=255) cgtaTAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:1509225/65‑1 (MQ=255) cGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:431105/68‑1 (MQ=255) cGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:2392106/68‑1 (MQ=255) cGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:419473/68‑1 (MQ=255) cGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:137839/68‑1 (MQ=255) cGGATAACTTCGCCATCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGga < 1:210099/68‑1 (MQ=255) tAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGaa > 1:485348/1‑69 (MQ=255) tAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGaa > 1:2074395/1‑69 (MQ=255) tAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGaa > 1:3201313/1‑69 (MQ=255) gTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCttt < 1:124733/69‑1 (MQ=255) gTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCttt > 1:2188347/1‑69 (MQ=255) gTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCttt < 1:836022/69‑1 (MQ=255) gTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCttt < 1:2871249/69‑1 (MQ=255) | AGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCTTT > minE/1629592‑1629722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |