| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,630,243 | G→A | 47.9% | R517R (CGC→CGT) | aegA ← | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,630,243 | 0 | G | A | 47.9% | 4.7 / 63.0 | 75 | R517R (CGC→CGT) | aegA | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (29/10); new base A (17/19); total (46/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGA > minE/1630182‑1630310 | cTTGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCg < 1:339798/68‑1 (MQ=255) tGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttc > 1:264890/1‑69 (MQ=255) tGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttc > 1:2981068/1‑69 (MQ=255) tGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttc > 1:2139373/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:863070/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:2558502/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:2904188/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:2269477/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:401648/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:519256/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:1814744/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:670821/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:727909/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:1742186/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:850422/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:1657341/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:829070/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt > 1:1580160/1‑68 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCACCCTCTTCACGGGCGttcttc > 1:380285/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGACCCCTCTTCACGGGCGttcttc > 1:2387837/1‑69 (MQ=255) tcattcaGTTCAAGCGCCACCCGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttcttc > 1:1817996/1‑69 (MQ=255) tcattcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttctt < 1:400841/68‑1 (MQ=255) cattcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttcttc < 1:1662201/68‑1 (MQ=255) cattcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttcttc < 1:2098476/68‑1 (MQ=255) cattcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttcttc < 1:3113134/68‑1 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:923832/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:2766331/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:849168/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:3099444/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:3127841/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:380642/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:801567/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:2455353/1‑69 (MQ=255) tcaGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCActt > 1:1688147/1‑69 (MQ=255) ccGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTg < 1:2689093/69‑1 (MQ=255) ccGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTg < 1:1982759/69‑1 (MQ=255) ccGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTg < 1:571234/69‑1 (MQ=255) ccGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTg < 1:3097179/69‑1 (MQ=255) cGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGtt < 1:2247366/67‑1 (MQ=255) cGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAg > 1:3040050/1‑69 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:734539/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:6030/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:1135685/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:1650084/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:855303/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:116121/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:1843713/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:868131/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:2002909/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:2028550/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:3433985/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:2067932/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:3306472/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:3263848/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:3261043/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:2944226/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:2191219/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCACGGGCGttctt < 1:2252180/38‑1 (MQ=255) ttAAATTCGAAGTTGGCCCCCTCTTCAC‑GGCGttctt < 1:1909060/37‑1 (MQ=37) cGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCac > 1:2924537/1‑69 (MQ=255) cGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCac > 1:2800903/1‑69 (MQ=255) cGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCac > 1:1542198/1‑69 (MQ=255) ggCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCa > 1:3383477/1‑47 (MQ=255) ggCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCa > 1:23057/1‑47 (MQ=255) ggCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCa > 1:420803/1‑47 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAgg > 1:821645/1‑68 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:2849108/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:2323554/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:2499524/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:1677870/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:1733013/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:2134350/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:3166818/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt > 1:3217749/1‑69 (MQ=255) ttCGCGGGCGTTCTTCACTTCTTTCTTCGAGACTGGCATGTTAGCTtcatca > 1:3265835/1‑52 (MQ=255) cgcgGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGa > 1:1203943/1‑69 (MQ=255) | CTTGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGA > minE/1630182‑1630310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |