| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,632,211 | C→T | 42.2% | R71R (CGC→CGT) | narQ → | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,632,211 | 0 | C | T | 42.2% | 44.8 / 87.1 | 119 | R71R (CGC→CGT) | narQ | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Reads supporting (aligned to +/- strand): ref base C (20/48); new base T (25/25); total (45/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTGGTATGTGCCAGA > minE/1632148‑1632276 | tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:1021479/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:704234/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:299563/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:2969316/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:2854063/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:2792352/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:2095904/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:1181673/68‑1 (MQ=255)tATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGACAGt < 1:1165085/68‑1 (MQ=255) aTGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:2633393/67‑1 (MQ=255) aCCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTa > 1:2515637/1‑59 (MQ=255) aCCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTAt > 1:11291/1‑60 (MQ=255) aCCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTAt > 1:2927260/1‑60 (MQ=255) aCCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTAt > 1:3020069/1‑60 (MQ=255) tGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGc < 1:127287/69‑1 (MQ=255) tGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGc < 1:74694/69‑1 (MQ=255) tGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGc < 1:1443832/69‑1 (MQ=255) tGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGc < 1:349484/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:2084381/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:945677/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:2533661/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1122125/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:2886664/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1044625/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:3234239/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:458494/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:533029/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:543678/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1024874/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:883572/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:916048/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1137258/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1186342/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1419423/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1435679/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc < 1:1373842/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGAAGGCACTGCATTCAc < 1:2790649/69‑1 (MQ=255) aCGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCATCAGTTATTTCAGCAGGCACTGCATTCAc < 1:3045159/69‑1 (MQ=255) cGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGt < 1:1903160/44‑1 (MQ=255) gACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCTGt < 1:1119351/43‑1 (MQ=255) aCTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCgg < 1:2975749/69‑1 (MQ=255) aCTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCgg < 1:3316019/69‑1 (MQ=255) aCTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCgg < 1:3432586/69‑1 (MQ=255) aCTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCgg < 1:2292876/69‑1 (MQ=255) aCTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCgg < 1:741735/69‑1 (MQ=255) aCTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCgg < 1:2572703/69‑1 (MQ=255) cTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCgg < 1:3278526/68‑1 (MQ=255) gTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaacca < 1:1982432/69‑1 (MQ=255) gTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaacca < 1:2417126/69‑1 (MQ=255) gTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaacca < 1:2701171/69‑1 (MQ=255) gTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaacca < 1:1669478/69‑1 (MQ=255) cAGTCGACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCAc > 1:1797805/1‑53 (MQ=255) cAGTCCACAACTCAATTCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:2239929/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCAtt > 1:1859130/1‑50 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:3008941/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:3162079/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:565580/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:3308696/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:3413033/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:3206234/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:368220/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:3263966/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:1069307/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:2088725/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:9358/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:907070/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:23028/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:2340904/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:1302202/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:2496267/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:2657647/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:2674891/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:657754/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaatc > 1:2934377/1‑66 (MQ=255) cAGTCCACAACTCAATGCACATCGTCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaaccaat > 1:1020463/1‑66 (MQ=255) gTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTaacca < 1:112227/63‑1 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAAc > 1:2973096/1‑68 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:44650/1‑69 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:586450/1‑69 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:2031981/1‑69 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:1675791/1‑69 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:3210643/1‑69 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:2734796/1‑69 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:3052595/1‑69 (MQ=255) cacaACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:3266261/1‑69 (MQ=255) aaCTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg < 1:1888809/66‑1 (MQ=255) aaCTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg > 1:234307/1‑66 (MQ=255) aaCTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg < 1:744245/66‑1 (MQ=255) aaCTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg < 1:581866/66‑1 (MQ=255) aaCTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg < 1:173775/66‑1 (MQ=255) aaCTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg < 1:1468098/66‑1 (MQ=255) aCTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg < 1:2449423/65‑1 (MQ=255) cTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAATGTCTgg < 1:1478077/69‑1 (MQ=255) cTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTgg < 1:1747405/69‑1 (MQ=255) cTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTgg < 1:1245715/69‑1 (MQ=255) cTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTgg < 1:2053253/69‑1 (MQ=255) tCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACg < 1:3183604/63‑1 (MQ=255) tGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTGGTAtg < 1:2115759/68‑1 (MQ=255) cacaTCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTGGTAtgt > 1:2228248/1‑67 (MQ=255) gCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTGGTATGTGCCAGa > 1:2605387/1‑67 (MQ=255) | TATGCAGAGTTACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCACCGGTATTAACCAACCTCAACGTCTGGTATGTGCCAGA > minE/1632148‑1632276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |