| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,632,688 | A→G | 31.9% | E230E (GAA→GAG) | narQ → | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,632,688 | 0 | A | G | 31.9% | 37.7 / 23.5 | 50 | E230E (GAA→GAG) | narQ | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Reads supporting (aligned to +/- strand): ref base A (7/27); new base G (7/9); total (14/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
GCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGT > minE/1632632‑1632746 | gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGTAGGCGTCAGTaga > 1:2830716/1‑68 (MQ=255)gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGc > 1:1900644/1‑59 (MQ=255)gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTaga > 1:117779/1‑68 (MQ=255)gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTaga > 1:2429224/1‑68 (MQ=255)gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTaga > 1:1502989/1‑68 (MQ=255)gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTaga > 1:3286138/1‑68 (MQ=255)gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTaga > 1:1805224/1‑68 (MQ=255) cTTTACCCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:911249/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:3122076/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:2874980/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:1748056/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:1709581/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:523552/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:634656/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:1186629/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAGGCGTCAGTAGAAGAAAAGAc < 1:1098605/69‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGt > 1:1623926/1‑53 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAc > 1:993811/1‑69 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAc > 1:3066159/1‑69 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAc > 1:22871/1‑69 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAc > 1:1675530/1‑69 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAc > 1:1896970/1‑69 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAc > 1:1496608/1‑69 (MQ=255) ccAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg < 1:2591717/68‑1 (MQ=255) cAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg < 1:1960322/67‑1 (MQ=255) cAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg < 1:2202776/67‑1 (MQ=255) cAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCcgcg < 1:3114247/67‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAACGACCCGCGAtctc < 1:3142464/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:3303094/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:1597091/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:3099964/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:2925288/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:1828871/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:2856125/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:1855381/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:2768512/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:929423/69‑1 (MQ=255) aTGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAtctc < 1:1903246/69‑1 (MQ=255) cTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:417075/42‑1 (MQ=255) cTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:1202878/42‑1 (MQ=255) cTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:937732/42‑1 (MQ=255) cTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:1063834/42‑1 (MQ=255) cTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:2329530/42‑1 (MQ=255) cTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:2014777/42‑1 (MQ=255) aaTTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:1376290/35‑1 (MQ=255) aaTTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:2351028/35‑1 (MQ=255) aaTTGTACCGTTCGCTGGAAGCGTCAGTAgaagaa < 1:1900138/35‑1 (MQ=255) ttGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTg < 1:1782688/69‑1 (MQ=255) ttGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTg < 1:2808970/69‑1 (MQ=255) gTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGgtgt < 1:343741/69‑1 (MQ=255) | GCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGT > minE/1632632‑1632746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |