| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,633,534 | T→C | 45.6% | G512G (GGT→GGC) | narQ → | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,633,534 | 0 | T | C | 45.6% | 11.0 / 75.7 | 90 | G512G (GGT→GGC) | narQ | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Reads supporting (aligned to +/- strand): ref base T (33/16); new base C (23/18); total (56/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGC > minE/1633479‑1633583 | gCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAAcc > 1:3435933/1‑67 (MQ=255)gCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAAcc > 1:3323721/1‑67 (MQ=255) cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:326766/69‑1 (MQ=255) cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:2516715/69‑1 (MQ=255) cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:1438784/69‑1 (MQ=255) cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:2520848/69‑1 (MQ=255) aCCGCGCCGTACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:3241011/1‑69 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGaaa < 1:556631/61‑2 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2568942/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2644226/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2974562/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:3033088/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:353217/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:37100/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2080436/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2058506/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2023913/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2001778/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:1963468/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:1013953/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:752040/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:892916/61‑1 (MQ=255) aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:1462525/61‑1 (MQ=255) aCCGCGCCGGATGACAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:166545/61‑1 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2016668/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2649093/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:1666209/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:1650083/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:3126119/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:944571/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:3093622/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2487172/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2913046/1‑69 (MQ=255) aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:97783/68‑1 (MQ=255) aCCGCGCC‑GATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:907889/60‑1 (MQ=255) tcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:2831842/2‑68 (MQ=255) gcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:768402/1‑68 (MQ=255) gcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:3408336/1‑68 (MQ=255) gcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:270501/1‑68 (MQ=255) cGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAg < 1:348808/69‑1 (MQ=255) acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:2768475/1‑68 (MQ=255) acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:1406361/1‑68 (MQ=255) acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:3014736/1‑68 (MQ=255) acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:645201/1‑68 (MQ=255) ttATATTCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:441647/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGaaa > 1:2301360/1‑40 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:1417266/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:3433130/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:2274134/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:1157487/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:742873/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:68106/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:3150190/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:3139942/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:1816704/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:195205/1‑41 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1063339/69‑1 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:252171/69‑1 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1837728/69‑1 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1787130/69‑1 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1306956/69‑1 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1088279/69‑1 (MQ=255) ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:3069985/69‑1 (MQ=255) tataTCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTg < 1:914314/63‑1 (MQ=255) tataTCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTg < 1:239041/63‑1 (MQ=255) tCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATgc > 1:1239349/1‑69 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:946550/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1173032/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:90068/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:994286/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:159683/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:767058/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1621864/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3095366/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1808971/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1857189/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:492753/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:404004/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2165866/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2253647/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2347877/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:246209/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:335600/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2985394/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3006792/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3244194/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3016622/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3034686/1‑53 (MQ=255) cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGGCTGAat > 1:1904761/1‑52 (MQ=255) gATTGGTATAGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTg < 1:3358390/46‑1 (MQ=255) | GCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGC > minE/1633479‑1633583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |