| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,634,474 | T→C | 41.6% | T204T (ACT→ACC) | acrD → | aminoglycoside/multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,634,474 | 0 | T | C | 41.6% | 18.1 / 42.7 | 60 | T204T (ACT→ACC) | acrD | aminoglycoside/multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base T (17/18); new base C (15/10); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCT > minE/1634406‑1634531 | tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCTACAGTTTCCAGATGACGGCTAAAGATGTCACt < 1:1118177/69‑1 (MQ=255)tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTATAGATGTCACt < 1:2466656/69‑1 (MQ=255)tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt < 1:2303234/69‑1 (MQ=255)tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt < 1:2258884/69‑1 (MQ=255)tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt < 1:209465/69‑1 (MQ=255)tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt < 1:3051411/69‑1 (MQ=255)tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt < 1:405067/69‑1 (MQ=255)tatTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt < 1:1216997/69‑1 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:506302/1‑69 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:3429509/1‑69 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:3027297/1‑69 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:1805219/1‑69 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:1879573/1‑69 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:2652909/1‑69 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:1957578/1‑69 (MQ=255) atTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg > 1:1239635/1‑69 (MQ=255) ccATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGAt > 1:417389/1‑68 (MQ=255) cGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACTGCTAAAGATGTCACTGAt > 1:64562/1‑63 (MQ=255) cGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa > 1:2981050/1‑67 (MQ=255) cGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa > 1:2574136/1‑67 (MQ=255) cGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa > 1:81553/1‑67 (MQ=255) cGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa > 1:842358/1‑67 (MQ=255) cGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa > 1:1378472/1‑67 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:2320532/65‑1 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:1509189/65‑1 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:3150310/65‑1 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:1620831/65‑1 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:1320911/65‑1 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:565514/65‑1 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:2667486/65‑1 (MQ=255) tggACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:2617359/65‑1 (MQ=255) ggCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCa < 1:612897/69‑1 (MQ=255) ggCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCa < 1:2816398/69‑1 (MQ=255) ggCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCa < 1:640212/69‑1 (MQ=255) ggCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCa < 1:2612180/69‑1 (MQ=255) gAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca < 1:2691793/55‑1 (MQ=255) cAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACgcgc > 1:717054/1‑59 (MQ=255) cAACAGCTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCa > 1:435103/1‑60 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCAt > 1:3072097/1‑41 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:621145/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:571131/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:420765/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:753954/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:367819/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:2274140/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:2075702/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:1752816/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:2971704/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:2630214/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:2793260/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:2911202/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCTAAAGATGTCACCGATGCCATTg > 1:2816401/1‑43 (MQ=255) aaCAGTTTCCAGATGACGGCT‑AAGATGTCACCGATGCCATTg > 1:66438/1‑42 (MQ=255) gACGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:1007092/69‑1 (MQ=255) gACGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:2572060/69‑1 (MQ=255) gACGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:1268375/69‑1 (MQ=255) gACGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:464943/69‑1 (MQ=255) gACGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:1593166/69‑1 (MQ=255) gACGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:412956/69‑1 (MQ=255) aCGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:476625/68‑1 (MQ=255) aCGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:1890727/68‑1 (MQ=255) aCGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:408148/68‑1 (MQ=255) aCGGCTAAAGATGTCACCGATGCCATTGAATCACAGAACGCGCAGATTGCGGTTGGGCAACTtggtgg < 1:1161825/68‑1 (MQ=255) ggCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTa < 1:3201018/68‑1 (MQ=255) aaaGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCt < 1:2833979/69‑1 (MQ=255) aaaGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCt < 1:724571/69‑1 (MQ=255) aaaGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCt < 1:138481/69‑1 (MQ=255) aaGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCt < 1:803432/68‑1 (MQ=255) | TATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCT > minE/1634406‑1634531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |