BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,639,502	A→G	53.9%	G179G (GGT→GGC)	ypfH ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,639,502	0	A	G	53.9%	24.9 / 98.1	123	G179G (GGT→GGC)	ypfH	predicted hydrolase
Reads supporting (aligned to +/- strand): ref base A (40/16); new base G (24/41); total (65/58)
Fisher's exact test for biased strand distribution p-value = 2.31e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

TTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTC  >  minE/1639442‑1639567

ttGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGc                                                           >  1:468576/1‑69 (MQ=255)

ttGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGc                                                           >  1:2938599/1‑69 (MQ=255)

ttGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGc                                                           >  1:1396403/1‑69 (MQ=255)

 tGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCa                                                          <  1:926792/69‑1 (MQ=255)

 tGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCa                                                          <  1:2031905/69‑1 (MQ=255)

  gCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCa                                                          >  1:1010454/1‑68 (MQ=255)

  gCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCa                                                          >  1:1201884/1‑68 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCTTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:2585207/69‑1 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCAGTGAtt                                                    <  1:196211/69‑1 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:953002/69‑1 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:2632185/69‑1 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:2250992/69‑1 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:2184949/69‑1 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:179986/69‑1 (MQ=255)

       cTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:1594518/69‑1 (MQ=255)

        tGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGAtt                                                    <  1:1248606/68‑1 (MQ=255)

         gCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTaa                                                  >  1:2523347/1‑69 (MQ=255)

         gCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTaa                                                  >  1:2590666/1‑69 (MQ=255)

         gCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTaa                                                  >  1:3383256/1‑69 (MQ=255)

         gCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTaa                                                  >  1:1893257/1‑69 (MQ=255)

         gCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTaa                                                  >  1:1860153/1‑69 (MQ=255)

            aTTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGc                                               >  1:2158737/1‑69 (MQ=255)

            aTTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGc                                               >  1:3247739/1‑69 (MQ=255)

            aTTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGc                                               >  1:2996548/1‑69 (MQ=255)

                     cGCATGGCCTAAATCCTCCACGATATCCAGCGTCTCGTCACCACCGGCACTGATTAACGCTTCCTgcgc                                      <  1:2829792/69‑1 (MQ=255)

                     cGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTgcgc                                      >  1:1040749/1‑69 (MQ=255)

                     cGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTgcgc                                      >  1:3251395/1‑69 (MQ=255)

                     cGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTgcgc                                      >  1:3059533/1‑69 (MQ=255)

                         tGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGcgccgc                                   >  1:3106437/1‑68 (MQ=255)

                         tGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCa                                  >  1:1009909/1‑69 (MQ=255)

                         tGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCa                                  >  1:3219893/1‑69 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAAc                                >  1:2010454/1‑69 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAAc                                >  1:567609/1‑69 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGACTAACGCTTCCTGCGCCGCAAc                                >  1:1989078/1‑69 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCacc                                >  1:2300829/1‑67 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCaa                                 >  1:2606451/1‑68 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCa                                  >  1:2304700/1‑67 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAAc                                >  1:1737462/1‑69 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAAc                                >  1:566509/1‑69 (MQ=255)

                           gCCTAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAAc                                >  1:1125362/1‑69 (MQ=255)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:310132/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:2975652/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:2967675/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:1034716/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:1575788/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:1474235/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:1793292/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:128739/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:2032976/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:2148866/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:1653523/48‑1 (MQ=38)

                              tAAATCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:1703907/48‑1 (MQ=38)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:1919510/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:1380896/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:1075316/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:3099586/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:1741903/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:1093280/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:168125/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:475935/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:2161785/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:2446439/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:2407511/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:2359178/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:560951/69‑1 (MQ=255)

                               aaaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:1587109/69‑1 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:524279/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:308498/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:1445685/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2290031/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCCCCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2215926/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCt                                              >  1:3223027/1‑50 (MQ=39)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACGGCAt                           >  1:3015005/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            >  1:3011034/1‑68 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            >  1:2952367/1‑68 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:1672212/68‑1 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCa                            <  1:488591/68‑1 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2274465/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2546147/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2543104/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:3192263/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:1110672/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2857124/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2857502/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:2931104/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCAt                           >  1:1902730/1‑69 (MQ=255)

                                aaTCCTCCACGATATCCAGCGTCACATCGCCACCGGCACTGATTAACGCTTCCTGCCCCGCAACCGCAt                           >  1:3271457/1‑69 (MQ=255)

                                   cctccACGATATCCAGCGTCACGTCACCACCGGGACTGATTAACGCTTCCTGCGCCGCAACCGCATgc                         >  1:3076616/1‑68 (MQ=255)

                                   cctccACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATgc                         >  1:1821874/1‑68 (MQ=255)

                                   cctccACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATgc                         >  1:1656388/1‑68 (MQ=255)

                                   cctccACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATgc                         >  1:313028/1‑68 (MQ=255)

                                    ctccACGATATCCAGCGTCACATCGCCACCGGCACTGATTaa                                                  <  1:2293015/42‑1 (MQ=38)

                                              tCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATa             <  1:582785/69‑1 (MQ=255)

                                                cAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAAc           >  1:2568496/1‑69 (MQ=255)

                                                cAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAAc           >  1:1093751/1‑69 (MQ=255)

                                                cAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAAc           >  1:318089/1‑69 (MQ=255)

                                                cAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATAGATAAc           >  1:819090/1‑69 (MQ=255)

                                                         gtcacCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTc  <  1:2370565/69‑1 (MQ=255)

                                                         gtcacCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTc  <  1:162373/69‑1 (MQ=255)

                                                          tcacCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTc  <  1:2755274/68‑1 (MQ=255)

TTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTGCGCCGCAACCGCATGCGCCAGATCGATAACCGGATCTTC  >  minE/1639442‑1639567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`