Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,642,040	C→G	31.2%	G30R (GGT→CGT)	ypfI ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,642,040	0	C	G	31.2%	72.5 / 43.2	66	G30R (GGT→CGT)	ypfI	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base G (10/10);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGTTAATGTG  >  minE/1642000‑1642104                                         |                                                                 aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:2086084/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:989769/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:563480/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:2560045/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:2378502/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:2370363/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:1927750/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:1777387/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:1643232/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:1341479/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:1139636/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:117990/63‑1 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcgg                                      >  1:1408796/1‑69 (MQ=255) aaGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcgg                                      >  1:1877282/1‑69 (MQ=255)  aGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:1391720/62‑1 (MQ=255)    gCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcggcg                                    <  1:1030423/68‑1 (MQ=255)    gCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGcggcgg                                   >  1:2772576/1‑69 (MQ=255)    gCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGcggcgg                                   >  1:1878745/1‑69 (MQ=255)    gCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGcggcgg                                   >  1:3420508/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:1030248/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:3184098/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:1871989/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:3219656/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:179890/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:2189996/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:1743822/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:2318830/1‑69 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATcc                               >  1:3207960/1‑68 (MQ=255)         aCGCAACTTAAGCGCATGATCAAAACACCAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATcc                               >  1:104013/1‑68 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCctct                                                              >  1:176648/1‑37 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCtt                                                             >  1:2158184/1‑38 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCtt                                                             >  1:1292127/1‑38 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCtt                                                             >  1:2016444/1‑38 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCtt                                                             >  1:1493518/1‑38 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcggc                                     >  1:2810027/1‑62 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:20011/1‑69 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:3326050/1‑69 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:623646/1‑69 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:2706120/1‑69 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:2587675/1‑69 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATcc                               >  1:1182739/1‑68 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATcc                               >  1:2625181/1‑68 (MQ=255)         aCGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATcc                               >  1:1833433/1‑68 (MQ=255)         aCGCAACTTAAGAGTATGCCCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATccc                              >  1:526580/1‑69 (MQ=255)                     agTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCa                   <  1:923642/68‑1 (MQ=255)                      gTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCa                   <  1:240182/67‑1 (MQ=255)                          gCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGc                                            <  1:634374/38‑1 (MQ=255)                                aaCACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGtt        <  1:1048151/68‑1 (MQ=255)                                aaCACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGtt        <  1:698598/68‑1 (MQ=255)                                aaCACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGtt        <  1:547969/68‑1 (MQ=255)                                 acacCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGTTaa      <  1:2446903/69‑1 (MQ=255)                                 acacCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGTTaa      <  1:2252370/69‑1 (MQ=255)                                 acacCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGTTaa      <  1:117967/69‑1 (MQ=255)                                    ccAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGTTAAtgt   >  1:1170437/1‑69 (MQ=255)                                    ccAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGTTAAtgt   >  1:1753396/1‑69 (MQ=255)                                     cAACTCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtatg  <  1:3149222/69‑1 (MQ=39)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCTGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:161332/69‑1 (MQ=39)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:1855341/69‑1 (MQ=255)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:3096133/69‑1 (MQ=255)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:290121/69‑1 (MQ=255)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:1731572/69‑1 (MQ=255)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:2827718/69‑1 (MQ=255)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:2010814/69‑1 (MQ=255)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:2752885/69‑1 (MQ=255)                                     cAACGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:1166710/69‑1 (MQ=255)                                      aaCGCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCAAGTTTCATTTGCGCTGTTAAtgtg  <  1:1680544/68‑1 (MQ=255)                                         ccTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCa                   <  1:652240/48‑1 (MQ=255)                                         |                                                                 AAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCGCTGTTAATGTG  >  minE/1642000‑1642104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑