| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,650,803 | A→G | 100% | R235R (CGT→CGC) | hda ← | ATPase regulatory factor involved in DnaA inactivation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,650,803 | 0 | A | G | 78.4% | 109.1 / 20.5 | 78 | R235R (CGT→CGC) | hda | ATPase regulatory factor involved in DnaA inactivation |
| Reads supporting (aligned to +/- strand): ref base A (16/1); new base G (61/0); total (77/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATT > minE/1650735‑1650863 | gccgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTa < 1:648661/69‑1 (MQ=255)gccgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTa < 1:2879680/69‑1 (MQ=255)gccgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTa < 1:2700897/69‑1 (MQ=255)gccgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTa < 1:2090460/69‑1 (MQ=255) ccgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCCTTCACAAACGGAATGGTCAGCTTa < 1:1651548/68‑1 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTgc > 1:3176559/1‑59 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGt > 1:1382905/1‑64 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATc > 1:1823325/1‑68 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATc > 1:1173262/1‑68 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATc > 1:2625321/1‑68 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATc > 1:1920685/1‑68 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:2327444/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:1783275/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:184771/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:1910099/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:1922601/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:2015073/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:2072844/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:252150/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:722005/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:728666/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:750395/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:939961/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:961580/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:1025188/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:1039699/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:1610786/1‑69 (MQ=255) aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCGTTGCGCGGTAATCg > 1:1229254/1‑69 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2640712/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2733622/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2730052/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1770714/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2740155/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2744221/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:276654/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2781321/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2819659/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2909963/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:3155812/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1289635/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:327460/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:412978/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:469020/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:594597/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:693416/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:709794/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1167604/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:879922/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2244055/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:170759/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1556143/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1951150/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1506491/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2076383/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1406858/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1365871/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2246543/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1379202/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2557611/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2551090/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:1003092/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2541422/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTGCg > 1:2525728/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa > 1:649687/1‑68 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc > 1:385194/1‑69 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc > 1:687369/1‑69 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc > 1:2162005/1‑69 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc > 1:1536533/1‑69 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCACGGTAATCGACGCAc > 1:1332726/1‑69 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCACCTTGCg > 1:2382570/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTAAGCTTGCg > 1:1198970/1‑49 (MQ=255) ttATCTACAACTTCAGAATTTCTTTAACAAACGGAATGGTCAGCTTGCg > 1:2521500/1‑49 (MQ=255) cAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAt > 1:1493101/1‑68 (MQ=255) cAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAt > 1:1618636/1‑68 (MQ=255) ttctttcACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGt < 1:1223176/69‑1 (MQ=255) tctttcACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCa > 1:2680515/1‑55 (MQ=255) tctttcACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCa > 1:2707017/1‑55 (MQ=255) tctttcACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCa > 1:660184/1‑55 (MQ=255) tctttcACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCa > 1:3403964/1‑55 (MQ=255) tctttcACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCa > 1:238862/1‑55 (MQ=255) gTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCAtt > 1:3053901/1‑69 (MQ=255) gTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCAtt > 1:1291075/1‑69 (MQ=255) gTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCAtt > 1:2786487/1‑69 (MQ=255) | GCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATT > minE/1650735‑1650863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |