| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,653,633 | C→A | 30.8% | intergenic (‑75/‑250) | upp ← / → purM | uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,653,633 | 0 | C | A | 30.8% | 146.0 / 64.6 | 110 | intergenic (‑75/‑250) | upp/purM | uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase |
| Reads supporting (aligned to +/- strand): ref base C (42/34); new base A (16/18); total (58/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTAT > minE/1653568‑1653699 | ccTTTGCGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:2190779/68‑1 (MQ=255)ccTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:1202595/68‑1 (MQ=255)ccTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:2483682/68‑1 (MQ=255)ccTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:290421/68‑1 (MQ=255)ccTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:1975352/68‑1 (MQ=255)ccTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:1009228/68‑1 (MQ=255)ccTTTGAGGAGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:1124414/68‑1 (MQ=255) cTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:807952/67‑1 (MQ=255) aGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTg > 1:510049/1‑69 (MQ=255) aGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTg > 1:794637/1‑69 (MQ=255) ggggCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTg < 1:1719433/68‑1 (MQ=255) ggggCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTg < 1:2717581/68‑1 (MQ=255) ggggCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTg < 1:2591074/68‑1 (MQ=255) ggCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAc > 1:1547240/1‑68 (MQ=255) gCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACtt < 1:695760/69‑1 (MQ=255) cAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACtt < 1:1286527/68‑1 (MQ=255) gCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt < 1:1760322/55‑1 (MQ=255) acaAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:1697165/69‑1 (MQ=255) acaAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:175607/69‑1 (MQ=255) acaAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:1564859/69‑1 (MQ=255) acaAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:1492196/69‑1 (MQ=255) acaAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:1242634/69‑1 (MQ=255) acaAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:397905/69‑1 (MQ=255) acaAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:1139216/69‑1 (MQ=255) caAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:2318899/68‑1 (MQ=255) caAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:2305837/68‑1 (MQ=255) caAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:2859567/68‑1 (MQ=255) caAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:3001463/68‑1 (MQ=255) caAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:39309/68‑1 (MQ=255) caAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:759746/68‑1 (MQ=255) aaaaaaaaTCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTATTTTCGTTGACTTTAGTCa < 1:2744108/67‑1 (MQ=255) cctTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGtt > 1:101069/1‑69 (MQ=255) ctTTCTTCAAGGCGGCAATATTCTTTTCGTTTACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTa > 1:1339990/1‑69 (MQ=255) ctTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGAt > 1:3255500/1‑62 (MQ=255) ctTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTa > 1:864304/1‑69 (MQ=255) ctTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTa > 1:1113871/1‑69 (MQ=255) ctTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTATAGTCAAAATGATAACGGTTTgaga > 1:1238421/1‑61 (MQ=255) gcggcAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa < 1:249093/69‑1 (MQ=255) gcggcAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa < 1:2014099/69‑1 (MQ=255) gcggcAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa < 1:761375/69‑1 (MQ=255) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:1127467/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:630634/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:489813/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:782434/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:3202717/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:3015076/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:2895547/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:2845901/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:1105345/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:1809610/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:2266857/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:1672685/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:1826198/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:191409/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:1497514/1‑39 (MQ=37) cggcAATATTATTTTCGTTGACTTTAGTCAAAACGATaa > 1:2300309/1‑39 (MQ=37) cAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATg < 1:200320/69‑1 (MQ=255) cAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATg < 1:72351/69‑1 (MQ=255) cAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATg < 1:338167/69‑1 (MQ=255) cAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATg < 1:2281219/69‑1 (MQ=255) atatTCTTTTCGTTGACTTTAGTCGAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:283682/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:2736954/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:2486730/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:1820058/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:79889/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:2444915/69‑1 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:1366351/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:54584/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:499320/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:1159613/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:3242882/69‑1 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:3241585/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:3154730/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:2821409/1‑69 (MQ=255) atatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:3013455/1‑69 (MQ=255) atatTCTTTTCCTTGACTTTAGTCAAAATGATAACGTTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:2701196/1‑69 (MQ=255) atatTCTTTTAGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt > 1:2052928/1‑69 (MQ=255) tatTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:3265840/68‑1 (MQ=255) atTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa < 1:1316041/61‑1 (MQ=255) atTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa < 1:2142294/61‑1 (MQ=255) atTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:2349568/67‑1 (MQ=255) atTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:3203523/67‑1 (MQ=255) atTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:149701/67‑1 (MQ=255) atTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:510445/67‑1 (MQ=255) atTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGt < 1:447016/67‑1 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa > 1:501350/1‑60 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa > 1:1247557/1‑60 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa > 1:1241903/1‑60 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCa > 1:795923/1‑60 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGAt > 1:1151831/1‑63 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:2961867/1‑68 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:1145078/1‑68 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:2594922/1‑68 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:1571938/1‑68 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:1485515/1‑68 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:3341624/1‑68 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:277088/1‑68 (MQ=255) ttCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTa > 1:3314571/1‑68 (MQ=255) ttCTTTTCGTTGACTTT‑GTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTAt > 1:1311385/1‑68 (MQ=255) ttCTTTTAGTTGACTTTAGTCAAAATGATAACGGTTTgaga > 1:1452500/1‑41 (MQ=255) | CCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTAT > minE/1653568‑1653699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |