| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,654,260 | T→C | 51.6% | I126I (ATT→ATC) | purM → | phosphoribosylaminoimidazole synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,654,260 | 0 | T | C | 51.6% | 0.4 / 53.0 | 62 | I126I (ATT→ATC) | purM | phosphoribosylaminoimidazole synthetase |
| Reads supporting (aligned to +/- strand): ref base T (7/23); new base C (14/18); total (21/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
CTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCC > minE/1654203‑1654319 | cTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGtt < 1:1961034/68‑1 (MQ=255)cTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGtt < 1:2171318/68‑1 (MQ=255)cTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGtt < 1:3100389/68‑1 (MQ=255) aCGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGc > 1:1098217/1‑69 (MQ=255) aCGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGc > 1:2053821/1‑69 (MQ=255) aCGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGc > 1:1368415/1‑69 (MQ=255) aCGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGc > 1:1338881/1‑69 (MQ=255) aCGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGc > 1:1222408/1‑69 (MQ=255) aCGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGc > 1:537446/1‑69 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:533837/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:3065052/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:2720461/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:758955/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:956039/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:1009093/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:963121/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:1862861/69‑1 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:1832311/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:1148331/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:1190546/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:642729/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:622617/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:451261/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:3374382/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:3087621/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:1301167/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:2998079/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:2686703/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:2403670/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:1563850/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:2307981/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:184041/69‑2 (MQ=255) aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:1413728/69‑2 (MQ=255) ccGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:2551581/68‑1 (MQ=255) gAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGtt < 1:2771220/54‑1 (MQ=255) aaCTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGtt < 1:2719768/69‑1 (MQ=255) aaCTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGtt < 1:120791/69‑1 (MQ=255) aaCTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGtt < 1:2995661/69‑1 (MQ=255) tGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:787441/59‑2 (MQ=255) tACCGCTTCAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcg < 1:1930603/49‑2 (MQ=39) aCCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGgtgggtgga < 1:1254876/69‑2 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:475694/68‑1 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:376433/68‑1 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:1448157/68‑1 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:116505/68‑1 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGGAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2927943/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATcgg > 1:2364642/1‑40 (MQ=38) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1647953/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:782039/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1246116/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1824735/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:3324030/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:409887/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:3392501/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2232599/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2291997/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1301203/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2632510/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2332358/1‑67 (MQ=255) ggTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:3013107/69‑1 (MQ=255) ggTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:2420300/69‑1 (MQ=255) cAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGcc > 1:837375/1‑69 (MQ=255) | CTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCC > minE/1654203‑1654319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |