| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,654,281 | A→G | 45.5% | S133S (TCA→TCG) | purM → | phosphoribosylaminoimidazole synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,654,281 | 0 | A | G | 45.5% | 4.5 / 43.9 | 55 | S133S (TCA→TCG) | purM | phosphoribosylaminoimidazole synthetase |
| Reads supporting (aligned to +/- strand): ref base A (11/19); new base G (13/12); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAC > minE/1654213‑1654344 | aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:963121/69‑1 (MQ=255)aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:956039/69‑1 (MQ=255)aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:758955/69‑1 (MQ=255)aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:533837/69‑1 (MQ=255)aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:3065052/69‑1 (MQ=255)aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:2720461/69‑1 (MQ=255)aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:1009093/69‑1 (MQ=255)aCCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:1862861/69‑1 (MQ=255) ccGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa < 1:2551581/68‑1 (MQ=255) aaCTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGtt < 1:2995661/69‑1 (MQ=255) aaCTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGtt < 1:120791/69‑1 (MQ=255) aaCTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGtt < 1:2719768/69‑1 (MQ=255) aCCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGgtgggtgga < 1:1254876/69‑2 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:1448157/68‑1 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:116505/68‑1 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:376433/68‑1 (MQ=255) gCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGa < 1:475694/68‑1 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGGAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2927943/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:409887/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2632510/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1246116/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:3324030/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:3392501/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2332358/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1301203/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2232599/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1647953/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:782039/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:2291997/1‑67 (MQ=255) cAGCGGTGATCAGCGGCATCGCGGAAGGTTGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAAc > 1:1824735/1‑67 (MQ=255) ggTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:2420300/69‑1 (MQ=255) ggTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:3013107/69‑1 (MQ=255) cAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGcc > 1:837375/1‑69 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:828471/53‑1 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:1708266/53‑1 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:673840/53‑1 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGa < 1:2084155/53‑1 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGcc > 1:393137/1‑59 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTa > 1:3111596/1‑68 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTAt > 1:3107818/1‑69 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTAt > 1:107914/1‑69 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTAt > 1:121884/1‑69 (MQ=255) gCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTAt > 1:2294011/1‑69 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACTGTGa < 1:958428/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:2523487/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:2497895/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:3409329/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:2444346/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:233567/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:1118381/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:1751732/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:1885840/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:826946/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:1949255/69‑1 (MQ=255) ttGTCTGCAATCGGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGa < 1:1150875/69‑1 (MQ=255) gTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAgg > 1:283121/1‑69 (MQ=255) gTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAgg > 1:1266144/1‑69 (MQ=255) gTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAgg > 1:452220/1‑69 (MQ=255) gTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAgg > 1:2780492/1‑69 (MQ=255) aTCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc < 1:1898464/67‑1 (MQ=255) aTCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc < 1:61218/67‑1 (MQ=255) aTCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc < 1:1960256/67‑1 (MQ=255) aTCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc < 1:2204569/67‑1 (MQ=255) aTCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc < 1:2883684/67‑1 (MQ=255) | ACCGGAAAACTGGATGTTGATACCGCTTCAGCGGTGATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAC > minE/1654213‑1654344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |