| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,655,091 | G→A | 62.1% | A58T (GCC→ACC) | purN → | phosphoribosylglycinamide formyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,655,091 | 0 | G | A | 62.1% | 38.5 / 50.9 | 79 | A58T (GCC→ACC) | purN | phosphoribosylglycinamide formyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base A (21/28); total (34/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01 | |||||||||||
AATAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGT > minE/1655025‑1655151 | aaTAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGcc < 1:2173565/69‑1 (MQ=255)aaTAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGcc < 1:1244578/69‑1 (MQ=255) aTAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGcc < 1:1511107/68‑1 (MQ=255) aaGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAgc > 1:2520467/1‑69 (MQ=255) aGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAgcg > 1:2692031/1‑69 (MQ=255) gACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGttt < 1:660343/69‑1 (MQ=255) ccTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTg < 1:1840009/68‑1 (MQ=255) cgcgCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTAt < 1:2742034/69‑1 (MQ=255) cgcgCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTAt < 1:2357024/69‑1 (MQ=255) cccgccAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGAcc < 1:264059/69‑1 (MQ=255) ccgccAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGAcc < 1:120670/68‑1 (MQ=255) ccgccAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGAcc < 1:2926926/68‑1 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAgc > 1:1099288/1‑38 (MQ=38) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTc > 1:2206733/1‑51 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:305062/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:3357031/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:1901885/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:31523/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:22821/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:34999/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:442839/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:2552039/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:154456/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:2623724/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:1319882/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:2719902/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:1202095/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:82364/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:847030/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCga > 1:1047758/1‑67 (MQ=255) gccAGGCGGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCga > 1:549114/1‑67 (MQ=255) gccAGGCGGGTATAGCAACACATACGCTCATCAcc > 1:2586273/1‑35 (MQ=25) gccAGGCCGGTATTGCAACACATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgag > 1:3352838/1‑67 (MQ=255) aGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAg > 1:2163584/1‑68 (MQ=255) ggCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGtt < 1:3011209/69‑1 (MQ=255) ggCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGtt < 1:1837509/69‑1 (MQ=255) gCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGAttg > 1:483755/1‑63 (MQ=255) gCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGaa > 1:1498652/1‑69 (MQ=255) gCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGaa > 1:1955184/1‑69 (MQ=255) gCAACGCATACGCTCATCGCCAGAGCGTTTGACAGTCGTGAAGc > 1:1759740/1‑44 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGaaa > 1:3293650/1‑65 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa < 1:1217433/69‑1 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa > 1:2327154/1‑69 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa < 1:2315334/69‑1 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa > 1:477755/1‑69 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa > 1:2057068/1‑69 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa < 1:2108643/69‑1 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa < 1:532996/69‑1 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa > 1:2244263/1‑69 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa < 1:2947627/69‑1 (MQ=255) gCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa > 1:1432672/1‑69 (MQ=255) cATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGAc < 1:1081023/69‑1 (MQ=255) cATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGAc < 1:313438/69‑1 (MQ=255) cATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGAc < 1:3420023/69‑1 (MQ=255) cATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGAc < 1:2492764/69‑1 (MQ=255) cATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGAc < 1:2397815/69‑1 (MQ=255) cATACGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGAc < 1:2772913/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:2060546/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:874088/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1133522/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1179281/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:662666/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1182433/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:541871/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1540870/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1599949/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1682964/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:2108653/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:215422/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:2194747/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:2255520/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:3185242/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:2291459/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:2353991/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:268881/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:2738675/69‑1 (MQ=255) cGCTCATCACCAGCGCGTTTGAAAGTCGTGAGGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1257993/69‑1 (MQ=255) gCTCATCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:1569337/68‑1 (MQ=255) aTCACCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGAGAGTTGATTCATGAAATCGACATGt < 1:562167/64‑1 (MQ=255) | AATAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGT > minE/1655025‑1655151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |