| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,657,322 | T→C | 72.0% | S531S (AGT→AGC) | ppk → | polyphosphate kinase, component of RNA degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,657,322 | 0 | T | C | 72.0% | 105.4 / 39.5 | 96 | S531S (AGT→AGC) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base T (9/18); new base C (36/33); total (45/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
CTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGT > minE/1657256‑1657377 | cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg > 1:2299615/1‑69 (MQ=255)cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg > 1:785441/1‑69 (MQ=255)cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg > 1:3039124/1‑69 (MQ=255)cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg > 1:3209531/1‑69 (MQ=255)cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg > 1:1572911/1‑69 (MQ=255)cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg > 1:1410758/1‑69 (MQ=255)cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg < 1:713941/69‑1 (MQ=255) tCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGt < 1:1402753/69‑1 (MQ=255) gccgccTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCa < 1:825714/69‑1 (MQ=255) gccgccTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCa < 1:2621787/69‑1 (MQ=255) gccgccTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCa < 1:2577504/69‑1 (MQ=255) gccgccTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCa < 1:1664296/69‑1 (MQ=255) gccgccTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCa < 1:1026363/69‑1 (MQ=255) gTATGAAATGGTGGACCGCGAGATAGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGc > 1:2675420/1‑68 (MQ=255) gTATGAAATGGTGGAC‑‑CGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTa > 1:1619275/1‑68 (MQ=255) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCTGTATCACCCTGAAGCTAAAt < 1:1297991/59‑1 (MQ=38) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCTGTATCACCCTGAAGCTAAAt < 1:1938562/59‑1 (MQ=38) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:2112694/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:2220352/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:199335/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:2293788/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:3394529/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:844739/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:2453252/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:3091592/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:647412/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:3085999/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:1130276/59‑1 (MQ=39) aCCGCGAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:516596/59‑1 (MQ=39) ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGa < 1:1982773/69‑1 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:300742/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:624458/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:1799385/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:3430817/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:367593/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:593911/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:40419/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:464025/1‑69 (MQ=255) cgAAATCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATaa > 1:1288114/1‑69 (MQ=255) aTCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGc < 1:1901476/69‑1 (MQ=255) aTCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGc < 1:1854658/69‑1 (MQ=255) aTCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGc < 1:1737815/69‑1 (MQ=255) aTCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGc < 1:388228/69‑1 (MQ=255) tCGCCAAGGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:638482/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:1044592/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:3424151/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:515427/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:478565/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:3092253/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:380156/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:3195569/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:3234189/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2168108/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:1129104/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:874986/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:1382249/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:1400575/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:1429378/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:1581062/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:1703267/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2071431/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2453728/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2480560/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:715780/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2733317/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2831448/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2928641/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2978043/1‑52 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCAGAAGCTAAATaa > 1:691821/1‑52 (MQ=39) tCGACAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATaa > 1:2230973/1‑52 (MQ=255) cAACGCGCAGCAATGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:1286744/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGTTATCACCCTGAAGCTAAAt < 1:1613983/46‑1 (MQ=39) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGTAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:3148114/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:586514/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:971400/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:836537/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:646465/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:528032/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:1327185/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:502627/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:3424195/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:3265788/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:2976667/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:2632953/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:2519973/69‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:2308612/69‑1 (MQ=255) aaCGCGCAGCAAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGt < 1:2803410/68‑1 (MQ=255) cagcaAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGc < 1:744915/57‑1 (MQ=255) gcaAGGGCTGCCCAGCGGTATCACCCTGAAGCTAAAt < 1:2225201/37‑1 (MQ=255) caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:701799/69‑1 (MQ=255) caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:1961646/69‑1 (MQ=255) caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:548816/69‑1 (MQ=255) caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:2699902/69‑1 (MQ=255) caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:586830/69‑1 (MQ=255) aaGGGCTGCCCAGCGGTATCACACTGAAGCTAAAt < 1:1078061/35‑1 (MQ=255) aGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGt > 1:1753866/1‑68 (MQ=255) | CTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGT > minE/1657256‑1657377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |