| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,657,763 | T→C | 33.3% | Y678Y (TAT→TAC) | ppk → | polyphosphate kinase, component of RNA degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,657,763 | 0 | T | C | 33.3% | 38.2 / 27.4 | 54 | Y678Y (TAT→TAC) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base T (13/23); new base C (8/10); total (21/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATCCCCTCGTC > minE/1657695‑1657828 | aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTTGCGATTTAt < 1:2394813/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:469343/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:2146006/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:1699587/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:506475/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:2703692/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:3134071/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:3280793/69‑1 (MQ=255)aaaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:3406157/69‑1 (MQ=255) aaGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTAt < 1:1832831/68‑1 (MQ=255) tCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaaca < 1:2723603/69‑1 (MQ=255) tCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaaca < 1:83372/69‑1 (MQ=255) tCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaaca < 1:526833/69‑1 (MQ=255) ccccGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaacaa < 1:129719/69‑1 (MQ=255) ccccGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaacaa < 1:2842271/69‑1 (MQ=255) ccccGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaacaa < 1:450173/69‑1 (MQ=255) gCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaaca < 1:810149/61‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:3182708/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:1004/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:3274065/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:2564504/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:1214108/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:128395/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:962748/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:1591926/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:164154/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:1929319/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:1653724/69‑1 (MQ=255) cgccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAAccc < 1:738982/69‑1 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:2017424/1‑69 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:1515356/1‑69 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:2659211/1‑69 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:2524963/1‑69 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:243363/1‑69 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:2357397/1‑69 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:2069128/1‑69 (MQ=255) gccgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCt > 1:563390/1‑69 (MQ=255) aGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCaa < 1:3332082/69‑1 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAAc > 1:735533/1‑46 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAAt > 1:2345523/1‑68 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAAt > 1:3180410/1‑68 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATc > 1:1630093/1‑69 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATc > 1:141558/1‑69 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATc > 1:2048388/1‑69 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATc > 1:3342183/1‑69 (MQ=255) ttGGCGATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATc > 1:1725961/1‑69 (MQ=255) tGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATcc > 1:2802205/1‑69 (MQ=255) tGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATcc > 1:2819703/1‑69 (MQ=255) tGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATcc > 1:1261082/1‑69 (MQ=255) ggCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATccc < 1:683416/69‑1 (MQ=255) ggCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATccc < 1:3121038/69‑1 (MQ=255) cGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATccc < 1:3186869/67‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:1110479/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:367643/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:2747951/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:263074/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:2303623/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:1700740/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:1695473/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:118958/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACCCTATGCCAATACACGATAAATCCCCt < 1:118082/68‑1 (MQ=255) gATTTACGACTACATCAAATCACTCGAACAATCTGAATAACACTATGCCAATACACGATAAATCCCCt < 1:2041565/68‑1 (MQ=255) ttATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATCCCCTCGTc > 1:2541728/1‑69 (MQ=255) ttATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATCCCCTCGTc > 1:1996592/1‑69 (MQ=255) | AAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATCCCCTCGTC > minE/1657695‑1657828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |