| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 130,273 | T→C | 37.0% | R151R (CGT→CGC) | yadE → | predicted polysaccharide deacetylase lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 130,273 | 0 | T | C | 37.0% | 44.8 / 50.6 | 81 | R151R (CGT→CGC) | yadE | predicted polysaccharide deacetylase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (30/21); new base C (16/14); total (46/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AATTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGG > minE/130208‑130334 | aaTTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGt > 1:2933541/1‑66 (MQ=255)aaTTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGt > 1:1279629/1‑66 (MQ=255) tGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCg < 1:118713/69‑1 (MQ=255) tACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGa > 1:2091811/1‑69 (MQ=255) aTTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGcc < 1:649080/69‑1 (MQ=255) aTTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGcc < 1:1017433/69‑1 (MQ=255) aTTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGcc < 1:68840/69‑1 (MQ=255) tttGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCta > 1:1622151/1‑69 (MQ=255) tttGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCt > 1:3317732/1‑68 (MQ=255) aGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:3029660/67‑1 (MQ=255) aGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:1687725/67‑1 (MQ=255) aGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:2875446/67‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:1896813/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:2791610/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:181427/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:1934173/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:1759986/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:1745395/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:2554413/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:614645/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:1692162/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:244011/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:2898610/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:678606/69‑1 (MQ=255) gACAGGTTAAATCAAACCTGGTATCAGATCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:2951794/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:1271423/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:263303/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:2053839/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:529372/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:1252250/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:3259275/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:64686/69‑1 (MQ=255) ggTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGccc < 1:1579322/69‑1 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGTGATCGACTGGCCTATATCAGCGCACTg < 1:2007297/59‑1 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg > 1:2855795/1‑59 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg > 1:3033763/1‑59 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg > 1:486301/1‑59 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:787609/59‑1 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg > 1:2290996/1‑59 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:1206409/59‑1 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:2740051/59‑1 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg > 1:1336944/1‑59 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg > 1:1427378/1‑59 (MQ=255) tAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:1466912/59‑1 (MQ=255) tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAAccc > 1:308057/1‑67 (MQ=255) tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCg > 1:1047159/1‑68 (MQ=255) tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCg > 1:116096/1‑68 (MQ=255) tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCg > 1:2440657/1‑68 (MQ=255) tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCg > 1:2079526/1‑68 (MQ=255) tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCg > 1:144731/1‑68 (MQ=255) tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCg > 1:1681967/1‑68 (MQ=255) gTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTg < 1:386599/46‑1 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTc > 1:12943/1‑67 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:3067800/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:1260023/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:1346405/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:1651314/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:1818761/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:600225/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:265488/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:2219335/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:3379014/1‑69 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCg > 1:429585/1‑68 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCg > 1:3222502/1‑68 (MQ=255) aGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGACCAACCCGATAATGGCCTGTCgg > 1:2471876/1‑69 (MQ=255) aGAACCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCgg > 1:1655647/1‑69 (MQ=255) tCCGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGAt < 1:2176763/41‑1 (MQ=38) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGAt > 1:2043754/1‑52 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:3051796/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:2901336/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:2832394/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:3353830/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:2392896/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:2369371/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:2323648/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:2133656/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:1924378/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:1724589/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:1549916/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:1384940/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:123388/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:1095118/1‑53 (MQ=39) ccGCATTGGCGATCGACTGGCCTATATCAGCGCGCTGGATGCCCAACCCGATa > 1:1019252/1‑53 (MQ=39) | AATTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGG > minE/130208‑130334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |