Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,658,023	C→A	38.5%	R75R (CGG→AGG)	ppx →	exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,658,023	0	C	A	38.5%	67.7 / 65.1	83	R75R (CGG→AGG)	ppx	exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base C (36/15);  new base A (21/11);  total (57/26)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTG  >  minE/1657962‑1658088                                                              |                                                                  taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa                                                            >  1:3242264/1‑69 (MQ=255) taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa                                                            >  1:1999967/1‑69 (MQ=255) taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa                                                            >  1:402269/1‑69 (MQ=255) taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa                                                            >  1:3356807/1‑69 (MQ=255) taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa                                                            >  1:375290/1‑69 (MQ=255) taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa                                                            >  1:471696/1‑69 (MQ=255) taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa                                                            >  1:2357037/1‑69 (MQ=255) taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCCGTTTGCCGAACGGCTACa                                                            >  1:395672/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:1997557/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:3002628/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:2018854/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:34805/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:2342997/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:3410855/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:2419473/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:2428471/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:335010/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:2792530/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:2932277/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:3086630/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:644237/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:1628431/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:1603556/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:1533218/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:102017/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:1353672/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:760350/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:535052/1‑69 (MQ=255)    tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg                                                         >  1:1206064/1‑69 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:513659/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:2777999/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:2765239/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:2668322/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:3353265/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:416021/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:2381426/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:2167437/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:2099548/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:46440/68‑1 (MQ=255)            gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct                                                  <  1:434141/68‑1 (MQ=255)                    gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAg                                          >  1:1337172/1‑68 (MQ=255)                    gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGc                                         >  1:1213176/1‑69 (MQ=255)                    gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGc                                         >  1:772720/1‑69 (MQ=255)                    gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGc                                         >  1:561023/1‑69 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:3272575/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:504314/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:869566/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:1064244/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:1153990/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:1176157/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:1198881/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:2376186/1‑56 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtct                                                  >  1:814379/1‑55 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:451110/1‑69 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:611168/1‑69 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:483980/1‑69 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:3152977/1‑69 (MQ=255)                         gACGCGCGGTTTAAACTGTCTGTCGATGTTTGCCGAACGGCTACAAGGGTTTtctc                                                 >  1:2035195/1‑56 (MQ=255)                            gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                 <  1:2307802/69‑1 (MQ=255)                            gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                 <  1:1127120/69‑1 (MQ=255)                               cgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGt                              <  1:1729977/69‑1 (MQ=255)                                ggTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt                             <  1:761084/69‑1 (MQ=255)                                ggTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt                             <  1:2987145/69‑1 (MQ=255)                                ggTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt                             <  1:633059/69‑1 (MQ=255)                                 gTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt                             <  1:3407798/68‑1 (MQ=255)                                 gTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt                             <  1:2934072/68‑1 (MQ=255)                                    tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:2444849/1‑58 (MQ=255)                                    tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:2555791/1‑58 (MQ=255)                                    tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:138203/1‑58 (MQ=255)                                    tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                    >  1:466326/1‑58 (MQ=255)                                           tctgtcGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAc                  >  1:1492696/1‑69 (MQ=255)                                                       tGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgc      >  1:473002/1‑69 (MQ=255)                                                       tGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgc      >  1:2455461/1‑69 (MQ=255)                                                       tGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgc      >  1:1317446/1‑69 (MQ=255)                                                       tGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgc      >  1:2515157/1‑69 (MQ=255)                                                       tGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgc      >  1:2121651/1‑69 (MQ=255)                                                           gAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg  <  1:3311298/69‑1 (MQ=255)                                                           gAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg  <  1:1675837/69‑1 (MQ=255)                                                           gAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg  <  1:1953915/69‑1 (MQ=255)                                                           gAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg  <  1:1072737/69‑1 (MQ=255)                                                           gAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg  <  1:3216877/69‑1 (MQ=255)                                                           gAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGAGCTg  <  1:2202058/69‑1 (MQ=255)                                                            aaCGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg  <  1:387804/68‑1 (MQ=255)                                                              |                                                                  TAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTG  >  minE/1657962‑1658088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑