| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,660,973 | G→T | 24.9% | L218I (CTC→ATC) | yfgF ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,660,973 | 0 | G | T | 24.9% | 221.5 / 60.0 | 132 | L218I (CTC→ATC) | yfgF | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (53/46); new base T (14/19); total (67/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
TTTTCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTA > minE/1660915‑1661034 | ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:3050008/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:1712178/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:1810507/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:739885/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:741080/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:1825925/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:598092/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:496725/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:413019/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:358847/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:347161/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:2706014/64‑1 (MQ=255)ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:2767580/64‑1 (MQ=255) ggCATGCATAACAGCAATAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGAc < 1:1667603/68‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:2281853/69‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:402822/69‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:3353533/69‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1805614/69‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1017782/69‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1636422/69‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1144348/69‑1 (MQ=255) tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1440342/69‑1 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:248219/56‑1 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:2372782/56‑1 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:2937113/56‑1 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:2237673/56‑1 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:675683/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2838105/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:1125128/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:253517/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:1209412/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2296602/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2884247/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2151139/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:1275840/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2008165/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:774824/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:611472/1‑68 (MQ=255) catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:3108693/1‑68 (MQ=255) atAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:673590/45‑1 (MQ=255) aaCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:2881877/69‑1 (MQ=255) aaCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:151686/69‑1 (MQ=255) aaCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:1736476/69‑1 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:2822347/1‑51 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:2083692/1‑51 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:1008702/1‑51 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:1030814/1‑51 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCaa > 1:1611819/1‑69 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCaa > 1:2879874/1‑69 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2415306/1‑68 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:865453/68‑1 (MQ=255) aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:2909898/68‑1 (MQ=255) gCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGCTGACTTTGGCATCAACct > 1:3161847/1‑69 (MQ=255) tAAAGCACCTAATGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACCTGCTGtt > 1:618787/1‑68 (MQ=255) tAAAGCACCTAATGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACCTGCTGtt > 1:3058766/1‑68 (MQ=255) tAAAGCACCTAATGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACCTGCTGtt > 1:1233808/1‑68 (MQ=255) tAAAGCACCTAATGCCAGAAGCCAGATTGCGAACTCTTTTTTGGt > 1:572495/1‑45 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:2369356/69‑1 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:3204566/69‑1 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1073442/69‑1 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:488819/69‑1 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1985655/69‑1 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1394112/69‑1 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:698414/69‑1 (MQ=255) aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1674663/69‑1 (MQ=255) aaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:3347195/68‑1 (MQ=255) aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:3421934/1‑55 (MQ=255) aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:97728/1‑55 (MQ=255) aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:2808050/1‑55 (MQ=255) aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:1891365/1‑55 (MQ=255) aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:276299/1‑55 (MQ=255) aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:2346295/1‑55 (MQ=255) aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCg > 1:1435460/1‑67 (MQ=255) aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:1511451/69‑1 (MQ=255) aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:2830383/69‑1 (MQ=255) aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:3276285/69‑1 (MQ=255) aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:2111107/69‑1 (MQ=255) aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGa > 1:2672859/1‑68 (MQ=255) aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGa > 1:3430600/1‑68 (MQ=255) aTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:425764/68‑1 (MQ=255) cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCa > 1:2954506/1‑40 (MQ=255) cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACctgatg > 1:3160898/1‑51 (MQ=255) cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaataat > 1:770735/1‑68 (MQ=255) cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAataata > 1:1029704/1‑69 (MQ=255) cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAataata > 1:1707436/1‑69 (MQ=255) cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAataata > 1:189861/1‑69 (MQ=255) gTAGCCAGAGCGAGAACTCTTTTTTGGTGACTTTgg < 1:184842/36‑1 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATc > 1:1999638/1‑37 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:3212930/1‑38 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2481012/1‑38 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2266237/1‑38 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:1548447/1‑38 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2685427/1‑38 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:3036051/38‑1 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:14264/1‑38 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:1247457/1‑38 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:3307676/1‑68 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:2337709/1‑68 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:1342782/1‑68 (MQ=255) gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:2662690/1‑68 (MQ=255) | TTTTCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTA > minE/1660915‑1661034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |