| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,661,722 | T→A | 13.5% | intergenic (‑98/‑254) | yfgF ← / → yfgG | predicted inner membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,661,722 | 0 | T | A | 13.5% | 295.8 / 32.8 | 140 | intergenic (‑98/‑254) | yfgF/yfgG | predicted inner membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (72/49); new base A (7/12); total (79/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
GTATTCAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGA > minE/1661662‑1661787 | gtATTCAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTa > 1:2739866/1‑69 (MQ=255)gtATTCAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTa > 1:43544/1‑69 (MQ=255) ttCAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAg > 1:2005771/1‑68 (MQ=255) cAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAgtgt > 1:2670375/1‑69 (MQ=255) cAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAgtgt > 1:631605/1‑69 (MQ=255) cAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAgtgt > 1:3167359/1‑69 (MQ=255) aCAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCat < 1:3002427/69‑1 (MQ=255) aCAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCa < 1:2227266/68‑1 (MQ=255) aCAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCa < 1:257307/68‑1 (MQ=255) aCAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCa < 1:1929045/68‑1 (MQ=255) aCAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCa < 1:3255978/68‑1 (MQ=255) cAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCat > 1:1020183/1‑68 (MQ=255) cAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCat > 1:1102984/1‑68 (MQ=255) aGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCata > 1:3323445/1‑68 (MQ=255) aGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCata > 1:558234/1‑68 (MQ=255) gCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCatat > 1:3162356/1‑67 (MQ=255) gCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCatat > 1:1741717/1‑67 (MQ=255) gCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCatat > 1:2247392/1‑67 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTTTCATATGaa < 1:1219624/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:2251853/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:2314811/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:2174909/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:782888/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:2778004/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:2956875/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:546476/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:3139566/69‑1 (MQ=255) cATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:177723/69‑1 (MQ=255) aTCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGaa < 1:2824811/68‑1 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGa > 1:684809/1‑47 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGa > 1:2700200/1‑47 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGa > 1:2794969/1‑47 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGa > 1:2603945/1‑47 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGa > 1:2530830/1‑47 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGa > 1:955761/1‑47 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGa > 1:2205730/1‑47 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAg > 1:610057/1‑67 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGt > 1:2279594/1‑68 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGt > 1:1596541/1‑68 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGt > 1:3197866/1‑68 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGt > 1:2189029/1‑68 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGt > 1:770034/1‑68 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGt > 1:72060/1‑68 (MQ=255) ccACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGt > 1:1347131/1‑68 (MQ=255) aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:2215753/69‑1 (MQ=255) aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:1165408/69‑1 (MQ=255) aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:1587108/69‑1 (MQ=255) aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:1890400/69‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGTATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:3046442/67‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:2391458/67‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:2387389/67‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:2959044/67‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:606454/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:459407/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:371513/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:356962/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:734586/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:3355631/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1582118/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:3313588/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:3303142/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:770323/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1662031/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1052008/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:201391/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:3001283/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:1881317/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1855118/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:2955270/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:2704925/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:2914231/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:2827740/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGat > 1:2638597/1‑68 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGat > 1:2081315/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:2303810/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:2784869/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:492052/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:2580227/1‑68 (MQ=255) caCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAAcc < 1:147193/69‑1 (MQ=255) aCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc > 1:1343539/1‑41 (MQ=255) aCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc > 1:1953407/1‑41 (MQ=255) aCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc > 1:1819856/1‑41 (MQ=255) tataGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:815661/39‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGACAGTTCCAATTGATATATATcaaacaa < 1:2112424/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2812153/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2828731/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:1490972/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2376396/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2081013/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2413524/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:3043937/57‑1 (MQ=255) gagagCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTa > 1:3036375/1‑69 (MQ=255) agagCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:312193/54‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:664682/69‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:365511/69‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:1795010/69‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:1499720/69‑1 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:3071271/1‑68 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:319754/1‑68 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:2889390/1‑68 (MQ=255) | GTATTCAGACAGGCATCCCACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGA > minE/1661662‑1661787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |