| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,661,747 | A→T | 24.6% | intergenic (‑123/‑229) | yfgF ← / → yfgG | predicted inner membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,661,747 | 0 | A | T | 24.6% | 128.8 / 39.2 | 81 | intergenic (‑123/‑229) | yfgF/yfgG | predicted inner membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (32/29); new base T (7/13); total (39/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCATGTTAATTCATC > minE/1661681‑1661815 | aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:1771667/69‑1 (MQ=255)aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:2215753/69‑1 (MQ=255)aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:1890400/69‑1 (MQ=255)aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:3023138/69‑1 (MQ=255)aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:1587108/69‑1 (MQ=255)aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:152155/69‑1 (MQ=255)aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:1165408/69‑1 (MQ=255)aCCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTATCATATGAAAGTAcc < 1:788074/69‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGTATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:3046442/67‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:2387389/67‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:2391458/67‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:622957/67‑1 (MQ=255) ttCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTg < 1:2959044/67‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:770323/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:2704925/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:2821524/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:2827740/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:2914231/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:2955270/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:3001283/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:606454/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:3303142/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1052008/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:3313588/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:3323430/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:734586/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:3355631/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:356962/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:371513/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:459407/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1582118/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1662031/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1832056/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:1855118/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata < 1:1881317/69‑1 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:201391/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGata > 1:2062487/1‑69 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGat > 1:2638597/1‑68 (MQ=255) tCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGat > 1:2081315/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:2303810/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:492052/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:3253692/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:3056759/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:2784869/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:2580227/1‑68 (MQ=255) cGAATGATGATTATTCATCACTATAGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGata > 1:2389644/1‑68 (MQ=255) caCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAAcc < 1:147193/69‑1 (MQ=255) aCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc > 1:1343539/1‑41 (MQ=255) aCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc > 1:1953407/1‑41 (MQ=255) aCTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc > 1:1819856/1‑41 (MQ=255) tataGAGAGCATTGATTCTAAGTGTCATATGAAAGTAcc < 1:815661/39‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGACAGTTCCAATTGATATATATcaaacaa < 1:2112424/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2199598/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2238047/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:1490972/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2376396/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2812153/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2413524/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2081013/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:2828731/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:268544/57‑1 (MQ=255) taGAGAGCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:3043937/57‑1 (MQ=255) gagagCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTa > 1:3036375/1‑69 (MQ=255) agagCAATGATTCTAAGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:312193/54‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:1795010/69‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:664682/69‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:365511/69‑1 (MQ=255) agCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATg < 1:1499720/69‑1 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:3071271/1‑68 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:1485435/1‑68 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:2889390/1‑68 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:748284/1‑68 (MQ=255) cATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGa > 1:319754/1‑68 (MQ=255) gATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAattat > 1:575497/1‑69 (MQ=255) gATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAattat > 1:312823/1‑69 (MQ=255) aGTGTCATATGAAAGTTCCAATTGATATATATcaaacaa < 1:40364/39‑1 (MQ=255) tCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCa > 1:3129152/1‑69 (MQ=255) tCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCa > 1:1299118/1‑69 (MQ=255) tGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCATGTTa > 1:138829/1‑69 (MQ=255) tGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCATGTTa > 1:1761679/1‑69 (MQ=255) tGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCATGTTa > 1:1033163/1‑69 (MQ=255) aCCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCATGTTAATtcatc > 1:2321733/1‑69 (MQ=255) | ACCTGACTTCGAATGATGATTATTCATCACTATAGAGAGCATTGATTCTAAGTGTCATATGAAAGTACCAATTGATATATATCAAACAAAATAACCCTGATTAATGAATTATTACGTTTATCATGTTAATTCATC > minE/1661681‑1661815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |