| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,669,173 | T→C | 33.7% | L290L (TTA→TTG) | der ← | predicted GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,669,173 | 0 | T | C | 33.7% | 60.7 / 47.8 | 86 | L290L (TTA→TTG) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (33/24); new base C (23/6); total (56/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
ACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCA > minE/1669107‑1669239 | aCTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTaa < 1:3418850/69‑1 (MQ=255) cTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAAc < 1:3241760/69‑1 (MQ=255) cTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAAc < 1:2668050/69‑1 (MQ=255) cTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAAc < 1:3157284/69‑1 (MQ=255) cTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAAc < 1:1226286/69‑1 (MQ=255) tAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGt > 1:3024284/1‑69 (MQ=255) tAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGt > 1:1532761/1‑69 (MQ=255) aaGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGtt > 1:2164864/1‑68 (MQ=255) aaGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGtt > 1:2583937/1‑68 (MQ=255) aaGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTg > 1:1387407/1‑69 (MQ=255) aaGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTg > 1:3323656/1‑69 (MQ=255) aaGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTg > 1:2266694/1‑69 (MQ=255) aaGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTg > 1:2379740/1‑69 (MQ=255) aaGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTg > 1:1521067/1‑69 (MQ=255) aaGCCCAGCAAGGAGAGATCCTGCTCGGAAATACCTTCACGCGCATCAATCACCAACATCACCACGTTg < 1:1156741/69‑1 (MQ=255) aaGCCCAGCAAGGAGAGATCCTGATCGGAAATACCTTCACGCGCATCAATCACCAACATCACCACGTTg < 1:3146449/69‑1 (MQ=255) aaGCCCAGCAAGGAGAGATCCTGATCGGAAATACCTTCACGCGCATCAATCACCAACATCACCACGTTg < 1:1401349/69‑1 (MQ=255) aaGCCCAGCAAGGAGAGATCCTGATCGGAAATACCTTCACGCGCATCAATCACCAACATCACCACGTTg < 1:731886/69‑1 (MQ=255) aaGCCCAGCAAGGAGAGATCCTGATCGGAAATACCTTCACGCGCATCAATCACCAACATCACCACGTTg < 1:644259/69‑1 (MQ=255) aaGCCCAGCAAGGAGAGATCCTGATCGGAAATACCTTCACGCGCATCAATCACCAACATCACCACGTTg < 1:1777925/69‑1 (MQ=255) cggcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTc < 1:520518/67‑1 (MQ=255) cagcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTc < 1:605537/69‑1 (MQ=255) cagcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTc < 1:2918929/69‑1 (MQ=255) cagcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTc < 1:3227659/69‑1 (MQ=255) agcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCa > 1:1494619/1‑69 (MQ=255) agcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCa < 1:927891/69‑1 (MQ=255) agcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCa > 1:979538/1‑69 (MQ=255) agcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCa < 1:2628248/69‑1 (MQ=255) agcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGGCTTCa > 1:35104/1‑69 (MQ=255) agcGAGAGATCCTGATCGGAAATACCTT‑G‑GCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAAt > 1:3058535/1‑69 (MQ=255) tCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGc < 1:2373563/69‑1 (MQ=255) ccTGATCGGTAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:1751492/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:1099076/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:2097334/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:1172896/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:1372577/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:1390880/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:896587/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:1479493/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:1604916/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:202901/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:2076694/1‑69 (MQ=255) ccTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCa > 1:3429840/1‑69 (MQ=255) aaTACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGAt < 1:3127768/69‑1 (MQ=255) tCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGgag < 1:3184760/69‑1 (MQ=255) tCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGgag < 1:2911198/69‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:73683/68‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:845824/68‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:586205/68‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:327261/68‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:99398/68‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:2570218/68‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:1437928/68‑1 (MQ=255) gcgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:2335284/68‑1 (MQ=255) cgcATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAAt < 1:60853/67‑1 (MQ=255) cacaAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:854865/1‑58 (MQ=255) caCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGc > 1:3297014/1‑69 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCTACGTTTTGATTACCGAGAAt > 1:2246838/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2097651/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2101558/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:1917780/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:174224/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:1717520/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:1653593/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:1405942/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:89812/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:1182688/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:946447/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:1179245/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:1109930/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2550128/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:3088995/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2992961/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2946436/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:3183086/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:3322966/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:3373974/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2147475/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2143884/1‑58 (MQ=255) caCCAACATCACCACGTTGGCATCTTCAATGGCCTGCAACGTTTTGATTACCGAGAAt > 1:2994412/1‑58 (MQ=255) aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTAGGGAGAATTTCTCTACAGCa > 1:917924/1‑69 (MQ=255) aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCa > 1:2340466/1‑69 (MQ=255) aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCa > 1:451722/1‑69 (MQ=255) aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCa > 1:3203595/1‑69 (MQ=255) aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCa > 1:3160876/1‑69 (MQ=255) aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCa > 1:2679746/1‑69 (MQ=255) aCTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCa > 1:2042130/1‑69 (MQ=255) | ACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCA > minE/1669107‑1669239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |