| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,671,075 | A→G | 37.5% | D88D (GAT→GAC) | yfgL ← | protein assembly complex, lipoprotein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,671,075 | 0 | A | G | 37.5% | 2.9 / 5.9 | 16 | D88D (GAT→GAC) | yfgL | protein assembly complex, lipoprotein component |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base G (2/4); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
AATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATA > minE/1671010‑1671122 | aaTGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCcatca < 1:661657/69‑1 (MQ=255)aaTGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCcatca < 1:2776689/69‑1 (MQ=255)aaTGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCcatca < 1:2363322/69‑1 (MQ=255) aTGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCcatca < 1:1526458/68‑1 (MQ=255) ggCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCa > 1:1632287/1‑69 (MQ=255) ggCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCa > 1:2269285/1‑69 (MQ=255) agaACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCt < 1:662928/68‑1 (MQ=255) agaACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCt < 1:2586411/68‑1 (MQ=255) gaACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCt < 1:1950265/67‑1 (MQ=255) tttCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAgc > 1:1146074/1‑69 (MQ=255) tttCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAgc > 1:2844672/1‑69 (MQ=255) tttCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAgc > 1:1678955/1‑69 (MQ=255) cTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCa < 1:803473/69‑1 (MQ=255) tGACAGACCAGATTTCTTTGCCGTCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTg > 1:2551009/1‑66 (MQ=255) tGACAGACCAGATTTCTTTGCCGTCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTg > 1:615905/1‑66 (MQ=255) gACAGACCAGATTTCTTTGCCGTCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATa < 1:139852/69‑1 (MQ=255) gACAGACCAGATTTCTTTGCCGTCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATa < 1:3050173/69‑1 (MQ=255) gACAGACCAGATTTCTTTGCCGTCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATa < 1:1154665/69‑1 (MQ=255) gACAGACCAGATTTCTTTGCCGTCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATa < 1:1106595/69‑1 (MQ=255) tttCTTTGACATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATa < 1:2026112/58‑1 (MQ=255) | AATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATA > minE/1671010‑1671122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |