| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 130,402 | A→C | 21.2% | T194T (ACA→ACC) | yadE → | predicted polysaccharide deacetylase lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 130,402 | 0 | A | C | 21.2% | 125.3 / 32.9 | 80 | T194T (ACA→ACC) | yadE | predicted polysaccharide deacetylase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (25/38); new base C (7/10); total (32/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
GCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCAT > minE/130336‑130467 | gCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATc < 1:1286490/69‑1 (MQ=255) cTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATc > 1:1605101/1‑68 (MQ=255) cTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATc > 1:3272445/1‑68 (MQ=255) cTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATc < 1:2905317/68‑1 (MQ=255) cTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATc > 1:2488748/1‑68 (MQ=255) aaCCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCgg < 1:2172833/68‑1 (MQ=255) tCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACgcgc > 1:2743308/1‑69 (MQ=255) tCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACgcgc > 1:1512310/1‑69 (MQ=255) atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAAt > 1:1240565/1‑69 (MQ=255) atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAAt > 1:2614673/1‑69 (MQ=255) atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAAt > 1:3326804/1‑69 (MQ=255) atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAAt > 1:3380479/1‑69 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAAt > 1:3391754/1‑68 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAAt > 1:1534354/1‑68 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATa > 1:272956/1‑69 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATa > 1:3235938/1‑69 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATa > 1:2522210/1‑69 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATa > 1:2346893/1‑69 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATa > 1:349019/1‑69 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATa > 1:1459874/1‑69 (MQ=255) ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATa > 1:1150690/1‑69 (MQ=255) gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCAATAACCAGATg > 1:1584683/1‑69 (MQ=255) gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCAATAACCAGATg > 1:1667882/1‑69 (MQ=255) gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCAATAACCAGATg > 1:1300801/1‑69 (MQ=255) gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCAATAACCAGATg > 1:508481/1‑69 (MQ=255) gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCAATAACCAGATg > 1:1243421/1‑69 (MQ=255) gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCAATAACCAGATg > 1:591785/1‑69 (MQ=255) gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCAATAACCAGATg > 1:728123/1‑69 (MQ=255) acgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATgg < 1:2420262/69‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:2343184/52‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:954500/52‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:2284677/52‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:495383/52‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:708823/52‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:864404/52‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:1872742/52‑1 (MQ=255) gaaAACACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:1083121/52‑1 (MQ=255) aaaaCACCCGTTTTCGCCATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:581207/51‑1 (MQ=255) aaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTg < 1:2026774/69‑1 (MQ=255) aaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTg < 1:3404537/69‑1 (MQ=255) aaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTg < 1:255573/69‑1 (MQ=255) acacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTg < 1:2247838/68‑1 (MQ=255) acacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTg < 1:1386298/68‑1 (MQ=255) cacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTg < 1:3400682/67‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:1740171/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:1267252/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:1503066/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:1677130/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:17403/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:3217443/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:1076127/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:3096555/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:3089362/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:1875435/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:2184431/69‑1 (MQ=255) acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt < 1:2102066/69‑1 (MQ=255) gTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACa < 1:294025/69‑1 (MQ=255) ttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAggg < 1:2616321/69‑1 (MQ=255) ttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAggg < 1:2581403/69‑1 (MQ=255) ttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAggg < 1:2170058/69‑1 (MQ=255) cGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAggg < 1:456703/67‑1 (MQ=255) ccATACTTCGACGACCACCTCGGTACGCGCTTTCa < 1:623705/35‑1 (MQ=255) cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGa > 1:1000150/1‑69 (MQ=255) cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGa > 1:822121/1‑69 (MQ=255) cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGa > 1:1087385/1‑69 (MQ=255) cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGa > 1:68552/1‑69 (MQ=255) cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGa > 1:1153258/1‑69 (MQ=255) cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGa > 1:348199/1‑69 (MQ=255) cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGa > 1:2263344/1‑69 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:350186/68‑1 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:2134426/68‑1 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:1621366/68‑1 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:1981798/68‑1 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:3130204/68‑1 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:2341435/68‑1 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:92007/68‑1 (MQ=255) tCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:2965260/68‑1 (MQ=255) gacCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAg < 1:1468562/69‑1 (MQ=255) gacCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAg < 1:2668608/69‑1 (MQ=255) acCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGac < 1:2133113/62‑1 (MQ=255) cacaTCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCAt > 1:42336/1‑69 (MQ=255) | GCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCAT > minE/130336‑130467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |