Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,676,325 T→C 46.5% V215V (GTA→GTG yfgB ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,676,3250TC46.5% 8.8 / 87.1 101V215V (GTA→GTGyfgBhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (43/11);  new base C (19/28);  total (62/39)
Fisher's exact test for biased strand distribution p-value = 8.10e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GCAGGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATG  >  minE/1676267‑1676388
                                                          |                                                               
gCAGGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAg                                                       <  1:1628782/69‑1 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:1483503/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:2388918/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:2359222/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:2307681/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:2034985/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:243206/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:1523912/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:514777/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:1313520/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:2776453/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:910506/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:3137017/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:3150453/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:1017359/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:3051806/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:1172282/1‑69 (MQ=255)
            ccAGCGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGa                                           >  1:2778971/1‑69 (MQ=255)
            ccAGCGAAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACAcc                                                            >  1:5322/1‑52 (MQ=38)
                 gCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGGACTACACCGGAAGTGGAAAGCGTGACGCGa                                      >  1:239989/1‑69 (MQ=255)
                 gCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGa                                      >  1:487735/1‑69 (MQ=255)
                 gCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGa                                      >  1:1230382/1‑69 (MQ=255)
                 gCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGa                                      >  1:774590/1‑69 (MQ=255)
                 gCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGa                                      >  1:2548115/1‑69 (MQ=255)
                 gCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGa                                      >  1:929845/1‑69 (MQ=255)
                 gCAACGTCGATCATATCGCCAAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGa                                      >  1:2218633/1‑69 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:457408/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:2780874/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:1419530/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:2359817/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:84443/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:1291373/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:560020/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:2053334/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:57397/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:1852821/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:2503462/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:173069/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:2520810/1‑67 (MQ=255)
                     cGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACg                                    >  1:2879877/1‑67 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCTGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:2480915/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:240887/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:354780/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:2543261/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:1961453/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:1941483/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:1144977/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:709516/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:1574198/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:1477040/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:968149/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:1392075/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:1302242/69‑3 (MQ=255)
                        cGATCATATCGCCCAGTTTATCCAGAGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGttttg                               <  1:2848478/69‑3 (MQ=255)
                            cATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGCCa                            >  1:1157922/1‑68 (MQ=255)
                            cATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAg                           >  1:1023677/1‑69 (MQ=255)
                            cATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAg                           >  1:553583/1‑69 (MQ=255)
                            cATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAg                           >  1:3056796/1‑69 (MQ=255)
                            cATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAg                           >  1:3399889/1‑69 (MQ=255)
                            cATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAg                           >  1:761236/1‑69 (MQ=255)
                            cATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAg                           >  1:657910/1‑69 (MQ=255)
                                tCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGcc                        >  1:12505/1‑68 (MQ=255)
                                tCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGcc                        >  1:1442159/1‑68 (MQ=255)
                                tCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGcc                        >  1:1762254/1‑68 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:852513/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:960775/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:800072/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:3370177/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:321536/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:968907/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:1210021/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:1245730/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:3054242/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:1352454/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:163257/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:2301036/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGTGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:2581098/68‑1 (MQ=255)
                                  gCCCAGTTTATCCAGCGCCGGAACCACACCGGAAGGGGAAAGCGTGACGCGACGTTTTGACAGGCCaa                      <  1:508412/68‑1 (MQ=255)
                                         ttATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGaa               >  1:3158569/1‑68 (MQ=255)
                                         ttATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGaa               >  1:1272385/1‑68 (MQ=255)
                                         ttATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGaa               >  1:1542233/1‑68 (MQ=255)
                                         ttATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGaa               >  1:1941205/1‑68 (MQ=255)
                                             ccAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAatca           >  1:3100498/1‑68 (MQ=255)
                                             ccAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAatca           >  1:125043/1‑68 (MQ=255)
                                                   ccGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGc     >  1:119020/1‑68 (MQ=255)
                                                   ccGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGc     >  1:2700451/1‑68 (MQ=255)
                                                   ccGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGc     >  1:1876224/1‑68 (MQ=255)
                                                   ccGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGc     >  1:3040630/1‑68 (MQ=255)
                                                   ccGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGc     >  1:2738107/1‑68 (MQ=255)
                                                   ccGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGc     >  1:2822949/1‑68 (MQ=255)
                                                    cGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCAt   <  1:1151863/69‑1 (MQ=255)
                                                    cGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCAt   <  1:527239/69‑1 (MQ=255)
                                                    cGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCAt   <  1:2510894/69‑1 (MQ=255)
                                                     ggAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCAt   <  1:2374713/68‑1 (MQ=255)
                                                     ggAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATg  <  1:3390573/69‑1 (MQ=255)
                                                     ggAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATg  <  1:751034/69‑1 (MQ=255)
                                                     ggAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATg  <  1:1775217/69‑1 (MQ=255)
                                                     ggAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATg  <  1:3160385/69‑1 (MQ=255)
                                                     ggAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATg  <  1:2688214/69‑1 (MQ=255)
                                                     ggAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATg  <  1:1177682/69‑1 (MQ=255)
                                                          |                                                               
GCAGGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATG  >  minE/1676267‑1676388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: