| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,677,347 | G→A | 39.1% | T68T (ACC→ACT) | ndk ← | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,677,347 | 0 | G | A | 39.1% | 51.8 / 63.6 | 97 | T68T (ACC→ACT) | ndk | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (26/33); new base A (22/16); total (48/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCA > minE/1677289‑1677407 | cgcgGTGACGCTGAACGGCGTTTTCACCTTCGAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:1683041/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:980430/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:2178319/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:2061394/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:2267659/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:2528038/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:2553414/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:1587276/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:3354367/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:73166/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:1083017/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:904004/1‑68 (MQ=255)cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAt > 1:810006/1‑68 (MQ=255) gcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTc < 1:2435394/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACTATCGGGCCAGAAGTCATGAATTc < 1:3041782/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGCTCGGGCCAGAAGTCATGAATTc < 1:3031122/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:594060/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:843453/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:1289881/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:2616573/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:1768475/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:2752079/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:2344932/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:1679948/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:632105/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:2086975/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:1496863/69‑1 (MQ=255) gcgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:543600/69‑1 (MQ=255) cgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:2789974/68‑1 (MQ=255) cgGTGACGCTGAACAGCATTTTCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTc < 1:29244/68‑1 (MQ=255) aCGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAg > 1:2840056/1‑69 (MQ=255) aCGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAg > 1:471833/1‑69 (MQ=255) gCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGAc < 1:2612174/69‑1 (MQ=255) gCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGAc < 1:1408027/69‑1 (MQ=255) gCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGAc < 1:298218/69‑1 (MQ=255) gCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGAc < 1:3319402/69‑1 (MQ=255) ttttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAg > 1:2580705/1‑66 (MQ=255) ttttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1392250/1‑69 (MQ=255) ttttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAAc > 1:3228295/1‑69 (MQ=255) ttttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1063473/1‑69 (MQ=255) ttttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1888597/1‑69 (MQ=255) ttttCACCTTCCAGCACGGAAACCACGATCGCGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1892499/1‑69 (MQ=255) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATg > 1:1639416/1‑45 (MQ=255) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCaa > 1:1394146/1‑52 (MQ=255) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGaa > 1:3071500/1‑68 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:2694234/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:2106531/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:2965126/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1676670/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:3340827/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1743617/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:158730/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1753476/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:2735909/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:2731804/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:750239/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1178728/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1917989/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:198181/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:1997996/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:3165620/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:944606/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATTCAACCAGACCATCAAAGAAc > 1:3171707/1‑69 (MQ=39) ttttCACCTTCCAGCACGGAAACAACGATCGGGCCAGAAGTCATGAATACAACCAGACCATCAAAGAAc > 1:2088350/1‑69 (MQ=255) ttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAg > 1:1752612/1‑54 (MQ=255) ttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAg > 1:2957229/1‑54 (MQ=255) ttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAg > 1:675036/1‑54 (MQ=255) ttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAg > 1:1909228/1‑54 (MQ=255) ttCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAg > 1:2159230/1‑54 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:1578457/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:453677/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:612313/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:1630514/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:1085080/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:225538/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:3116304/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:2259250/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:1909418/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:279084/68‑1 (MQ=255) ccTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:2977265/68‑1 (MQ=255) cTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGttt < 1:1074139/67‑1 (MQ=255) cTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTc < 1:1656029/69‑1 (MQ=255) cTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTc < 1:1455161/69‑1 (MQ=255) cTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTc < 1:3138318/69‑1 (MQ=255) ttCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTc < 1:2531597/68‑1 (MQ=255) cACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCgtg < 1:149183/69‑1 (MQ=255) cACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCgtg < 1:156878/69‑1 (MQ=255) cACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCgtg < 1:3153910/69‑1 (MQ=255) cACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCgtg < 1:1297739/69‑1 (MQ=255) cACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCgtg < 1:3311926/69‑1 (MQ=255) cACGGAAACCACGATCGGGCCAGAGGTCATAAATTCAACCAGACCATCAAAGAACGGTTTTCCATCgtg < 1:716009/69‑1 (MQ=255) aaaCCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCgtg < 1:3400386/64‑1 (MQ=255) gggCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAGGCCa < 1:292032/69‑1 (MQ=255) gggCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCa < 1:2991170/69‑1 (MQ=255) gggCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCa < 1:389690/69‑1 (MQ=255) gggCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCa < 1:796862/69‑1 (MQ=255) gggCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCa < 1:3017919/69‑1 (MQ=255) | CGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCA > minE/1677289‑1677407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |