| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,677,494 | G→C | 51.9% | V19V (GTC→GTG) | ndk ← | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,677,494 | 0 | G | C | 51.9% | 5.3 / 94.3 | 79 | V19V (GTC→GTG) | ndk | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (29/9); new base C (19/22); total (48/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
AGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTACCTCTG > minE/1677426‑1677561 | aGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATg < 1:2188969/69‑1 (MQ=255)aGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATg < 1:802414/69‑1 (MQ=255) gTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGAc > 1:3430328/1‑69 (MQ=255) gTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGAc > 1:2933903/1‑69 (MQ=255) tGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:125211/1‑69 (MQ=255) tGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:2273960/1‑69 (MQ=255) tGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:2708920/1‑69 (MQ=255) tGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:1328800/1‑69 (MQ=255) tGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:1402522/1‑69 (MQ=255) tGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:1487889/1‑69 (MQ=255) tGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:3343596/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGCCGt > 1:1507810/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACg > 1:2543781/1‑68 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt > 1:2535205/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt > 1:2403358/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt > 1:311800/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt > 1:1960778/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt < 1:188217/69‑1 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt > 1:1017816/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt > 1:524424/1‑69 (MQ=255) gcagcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGt > 1:1189148/1‑69 (MQ=255) gcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGtttt > 1:420252/1‑69 (MQ=255) gcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGtttt > 1:254317/1‑69 (MQ=255) gcaTTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGtttt > 1:1809595/1‑69 (MQ=255) tttgTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2681815/4‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGc > 1:1154584/1‑68 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2705591/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2785385/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2914142/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2486354/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2921944/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:293515/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2113284/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:314740/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:1577731/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:1436286/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:3195797/1‑69 (MQ=255) ttGGTGCCGACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCt > 1:2106367/1‑69 (MQ=255) ttGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGc > 1:1246381/1‑68 (MQ=255) ccAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACcgcg > 1:2701101/1‑69 (MQ=255) ccAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACcgcg > 1:2289291/1‑69 (MQ=255) ccAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACcgcg > 1:2554825/1‑69 (MQ=255) caaTTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCg > 1:1307157/1‑69 (MQ=255) caaTTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCg > 1:1622639/1‑69 (MQ=255) caaTTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCg > 1:553566/1‑69 (MQ=255) caaTTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCg > 1:3208004/1‑69 (MQ=255) caaTTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCg > 1:131195/1‑69 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:3217147/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:423649/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:809393/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:36367/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:2744907/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:936384/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:417016/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:3151051/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:3032204/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:2657581/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:1688215/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:1132268/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:1893267/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:1953831/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:1974717/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:1987740/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:2026403/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:210093/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:2447676/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:2459526/68‑1 (MQ=255) aCCCTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCCCGTTCGGTTtgatg < 1:2575595/68‑1 (MQ=255) cccTGCAGCTTCAAAGCGCGCAAAGATACTACCAATCACGTTTTTTGCTACCGCGTTCGGTTtgatg < 1:1657031/67‑1 (MQ=255) ccTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTgatgat > 1:2538209/1‑68 (MQ=255) gCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTCCCGCGtt > 1:1372964/1‑53 (MQ=255) cAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGa > 1:15723/1‑67 (MQ=255) gcgcgcAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTc < 1:2143788/69‑1 (MQ=255) gcgcgcAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTc < 1:901467/69‑1 (MQ=255) gcgcgcAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTc < 1:2284113/69‑1 (MQ=255) cgcgcAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTc < 1:3348606/68‑1 (MQ=255) ttACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCAttttt > 1:1285311/1‑69 (MQ=255) ttACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCAttttt < 1:1094813/69‑1 (MQ=255) ttACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCAttttt < 1:1735547/69‑1 (MQ=255) ttACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCAttttt < 1:1787871/69‑1 (MQ=255) cAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTAcc < 1:2254522/68‑1 (MQ=255) gACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTACCTCTg > 1:1502718/1‑68 (MQ=255) gACGTTTTTTGCTAACGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTACCTCTg > 1:2631666/1‑68 (MQ=255) | AGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTACCTCTG > minE/1677426‑1677561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |