| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 130,742 | G→A | 52.9% | V308I (GTA→ATA) | yadE → | predicted polysaccharide deacetylase lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 130,742 | 0 | G | A | 52.9% | 3.5 / 72.0 | 84 | V308I (GTA→ATA) | yadE | predicted polysaccharide deacetylase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (22/17); new base A (14/30); total (36/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTG > minE/130675‑130799 | cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCggg < 1:1569650/68‑1 (MQ=255)cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCggg < 1:595455/68‑1 (MQ=255)cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCggg < 1:2679868/68‑1 (MQ=255)cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCggg < 1:3241396/68‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCTTCGGGTa < 1:2985925/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:2350739/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:2098699/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:600879/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:2015829/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:580024/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:1645799/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:1394758/69‑1 (MQ=255) gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:3002125/69‑1 (MQ=255) tttCTGATCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGTTa < 1:2599708/68‑1 (MQ=255) tCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGAt > 1:1591929/1‑69 (MQ=255) tCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGAt > 1:1630050/1‑69 (MQ=255) tCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGAt > 1:3151831/1‑69 (MQ=255) tCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGAt > 1:421563/1‑69 (MQ=255) tCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGAt > 1:483996/1‑69 (MQ=255) tCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGAt > 1:766881/1‑69 (MQ=255) cTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAt > 1:786711/1‑69 (MQ=255) cTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAt > 1:1562810/1‑69 (MQ=255) cTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAt > 1:1520027/1‑69 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:3016733/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:3122126/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:3425511/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:354193/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:2473046/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:2427474/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:1172731/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:2086689/69‑1 (MQ=255) ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACTCATTTTTTGCATCGGATAGATGGTTATc < 1:2294374/69‑1 (MQ=255) cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTa < 1:532787/55‑1 (MQ=255) cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:2869332/1‑69 (MQ=255) cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:635098/1‑69 (MQ=255) cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:2405231/1‑69 (MQ=255) cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:995058/1‑69 (MQ=255) cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:2148885/1‑69 (MQ=255) cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:1257080/1‑69 (MQ=255) cGAAATTCGCGATGTATTTGATTT‑CAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGa > 1:3050963/1‑69 (MQ=255) aTTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATGGGGTAGATGGTTATCGCCGAccc < 1:2979932/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1259238/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1348653/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1433423/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1573818/69‑1 (MQ=255) gATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1915540/64‑1 (MQ=255) tttGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc > 1:2620451/1‑69 (MQ=255) aTTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGt < 1:3066171/68‑1 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTg > 1:2452447/1‑68 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1466739/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:309454/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:2388706/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:197627/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1049846/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1300204/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1940560/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1761935/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3256514/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3279268/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1342739/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1079912/1‑69 (MQ=255) aTTTTTTGCATAGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3074273/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGa > 1:1123344/1‑42 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:1659862/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:473200/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:550340/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:2822523/1‑69 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1328291/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3055156/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:93002/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:108989/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:714824/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1049094/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1385321/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3413015/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1513064/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:229415/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3001774/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1739439/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2930983/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2920247/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2783278/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1876398/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2677249/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2676678/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2506743/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:216183/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2244063/48‑1 (MQ=255) | CGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTG > minE/130675‑130799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |