| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,677,674 | T→A | 28.5% | intergenic (‑124/+25) | ndk ← / ← pbpC | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase/fused transglycosylase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,677,674 | 0 | T | A | 28.5% | 90.8 / 42.1 | 70 | intergenic (‑124/+25) | ndk/pbpC | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase/fused transglycosylase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (43/7); new base A (9/11); total (52/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.17e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCG > minE/1677608‑1677726 | ttATAATGGGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGtttt > 1:499679/1‑67 (MQ=255)ttATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGtttt > 1:501978/1‑67 (MQ=255)ttATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTa > 1:242154/1‑68 (MQ=255)ttATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTa > 1:87949/1‑68 (MQ=255)ttATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTa > 1:1700574/1‑68 (MQ=255)ttATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTa > 1:3213578/1‑68 (MQ=255)ttATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTa > 1:1493715/1‑68 (MQ=255) taATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGca > 1:2412538/1‑68 (MQ=255) taATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGca > 1:1807691/1‑68 (MQ=255) aTGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGcaaca > 1:2605840/1‑69 (MQ=255) aTGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGcaaca > 1:486758/1‑69 (MQ=255) gCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACaa > 1:1874340/1‑68 (MQ=255) gCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACaa > 1:3197506/1‑68 (MQ=255) ccGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACaaaa < 1:1715733/69‑1 (MQ=255) gACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACaaaa > 1:174993/1‑64 (MQ=255) gACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACaaaa > 1:103068/1‑64 (MQ=255) gACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACaaaa > 1:1276253/1‑64 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCa > 1:502673/1‑50 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2571313/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2608655/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2361510/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2611400/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2631369/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:484968/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:578824/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:591352/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:89549/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:924977/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:998326/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1739876/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1347888/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1244947/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1516320/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:114106/1‑69 (MQ=255) ttGATCAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1768997/1‑69 (MQ=255) gatgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGc > 1:2911595/1‑68 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTg > 1:2634608/1‑66 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCa > 1:3067123/1‑68 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:3040194/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:1947377/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:877961/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:2708636/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:738721/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:1129687/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:455723/1‑69 (MQ=255) gaAGGTAAGATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1304734/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAATAATCAGGGACAAAATAGACTATTGCATg < 1:1373284/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1442507/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1396696/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1170142/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2270984/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:519358/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2712709/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2477835/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1041968/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2385201/68‑1 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCa > 1:3133769/1‑38 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAg > 1:1030701/1‑39 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAttt > 1:319035/1‑69 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAttt > 1:289673/1‑69 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAttt > 1:1729967/1‑69 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAtt > 1:504789/1‑68 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:3331267/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:3123394/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:2766049/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:2064139/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa > 1:2747902/1‑69 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa > 1:2146993/1‑69 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTAATGCATGACAAATTTc < 1:1362472/68‑1 (MQ=255) tttttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCAc > 1:2811241/1‑69 (MQ=255) tttttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCAc > 1:2076212/1‑69 (MQ=255) aaataaaatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTcgcg < 1:656741/68‑1 (MQ=255) | TTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCG > minE/1677608‑1677726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |