Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,677,695 | C→T | 38.4% | intergenic (‑145/+4) | ndk ← / ← pbpC | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase/fused transglycosylase and transpeptidase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,677,695 | 0 | C | T | 38.4% | 26.2 / 31.2 | 52 | intergenic (‑145/+4) | ndk/pbpC | multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase/fused transglycosylase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base C (26/6); new base T (9/11); total (35/17) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.37e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
TTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCG > minE/1677636‑1677726 | ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2631369/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:998326/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:924977/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:89549/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:591352/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:578824/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:484968/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2611400/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2608655/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2571313/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:2361510/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1739876/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1516320/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:114106/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1244947/1‑69 (MQ=255) ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1347888/1‑69 (MQ=255) ttGATCAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTg > 1:1768997/1‑69 (MQ=255) gatgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGc > 1:2911595/1‑68 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTg > 1:2634608/1‑66 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCa > 1:3067123/1‑68 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:1129687/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:877961/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:738721/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:455723/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:3040194/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:2708636/1‑69 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCAt > 1:1947377/1‑69 (MQ=255) gaAGGTAAGATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1304734/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAATAATCAGGGACAAAATAGACTATTGCATg < 1:1373284/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2477835/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:519358/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2270984/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1442507/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1041968/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2385201/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1396696/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:1170142/68‑1 (MQ=255) gaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTATTGCATg < 1:2712709/68‑1 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAttt > 1:289673/1‑69 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAttt > 1:319035/1‑69 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAttt > 1:1729967/1‑69 (MQ=255) aCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAtt > 1:504789/1‑68 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:3331267/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:2064139/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:3123394/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTc < 1:2766049/68‑1 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa > 1:2146993/1‑69 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa > 1:2747902/1‑69 (MQ=255) aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTAATGCATGACAAATTTc < 1:1362472/68‑1 (MQ=255) tttttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCAc > 1:2076212/1‑69 (MQ=255) tttttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCAc > 1:2811241/1‑69 (MQ=255) aaataaaatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTcgcg < 1:656741/68‑1 (MQ=255) | TTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCG > minE/1677636‑1677726 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |