| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,678,452 | G→A | 68.0% | D520D (GAC→GAT) | pbpC ← | fused transglycosylase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,678,452 | 0 | G | A | 68.0% | 62.6 / 39.3 | 78 | D520D (GAC→GAT) | pbpC | fused transglycosylase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base G (17/8); new base A (47/6); total (64/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
CCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGC > minE/1678394‑1678511 | ccGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGc < 1:635492/69‑1 (MQ=255)ccGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGc < 1:881197/69‑1 (MQ=255) ctgTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:465768/3‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGc > 1:52160/1‑68 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGc > 1:3192956/1‑68 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGc < 1:93572/68‑1 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGc > 1:3046718/1‑68 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGc > 1:197584/1‑68 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:687311/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:701214/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:454075/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:1968917/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:1972280/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:658030/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:2801753/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:553840/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:2192210/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:505163/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:172508/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:16721/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:829546/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:1548323/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:3091008/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:1482725/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:3395846/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:1329224/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:1266402/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:1216802/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:10958/1‑67 (MQ=255) cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAg > 1:3295918/1‑67 (MQ=255) gTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCAt < 1:2848161/69‑1 (MQ=255) cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGGCCAGGCGTCACGATAGCCATAg > 1:2055272/1‑69 (MQ=255) cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAg > 1:238570/1‑69 (MQ=255) cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAg > 1:1301527/1‑69 (MQ=255) cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAg > 1:1339231/1‑69 (MQ=255) cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAg > 1:1903186/1‑69 (MQ=255) cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAg > 1:1872857/1‑69 (MQ=255) cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAg > 1:428710/1‑69 (MQ=255) gATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCg < 1:2732165/68‑1 (MQ=255) aTGACATAGCGAGCGTTACCCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:3107847/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATa > 1:2672535/1‑50 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATa > 1:915083/1‑50 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGc > 1:2119397/1‑58 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:35401/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:3420432/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:856189/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:941545/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:546985/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:3391137/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:52735/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:10557/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:2369824/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:1094632/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:1810712/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:1980917/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:2112903/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:2148334/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:3351367/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:238392/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:2728083/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:3183254/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:3032872/1‑69 (MQ=255) aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCAt > 1:2983799/1‑69 (MQ=255) aaCCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGtt > 1:2761095/1‑46 (MQ=255) aaCCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGAcgcg > 1:171393/1‑69 (MQ=255) ccAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGc < 1:2881047/69‑1 (MQ=255) cAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGc < 1:1627843/68‑1 (MQ=255) cGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc > 1:3145353/1‑69 (MQ=255) cGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc > 1:2683258/1‑69 (MQ=255) cGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc > 1:871241/1‑69 (MQ=255) cGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc > 1:1417462/1‑69 (MQ=255) gCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc < 1:402861/68‑1 (MQ=255) gCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc < 1:2940817/68‑1 (MQ=255) gCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc < 1:3398498/68‑1 (MQ=255) gCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc < 1:725224/68‑1 (MQ=255) gCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc < 1:1667646/68‑1 (MQ=255) gCCCAGGCATCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGc < 1:1534101/68‑1 (MQ=255) ccAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGc < 1:1542059/61‑1 (MQ=255) | CCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGC > minE/1678394‑1678511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |