| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,679,130 | C→T | 40.9% | R294R (CGG→CGA) | pbpC ← | fused transglycosylase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,679,130 | 0 | C | T | 40.9% | 32.9 / 53.1 | 78 | R294R (CGG→CGA) | pbpC | fused transglycosylase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (8/38); new base T (15/17); total (23/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
ACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCC > minE/1679076‑1679184 | acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:3015094/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:560807/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:529958/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:2042763/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:575061/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1822558/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1717184/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:616519/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1645727/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1536038/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:821813/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:27470/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:2960034/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:884073/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1480869/69‑1 (MQ=255)acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:858003/69‑1 (MQ=255) tCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGcc > 1:2117483/1‑69 (MQ=255) aTCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGttct > 1:2459389/1‑67 (MQ=255) aTCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGttct > 1:597508/1‑67 (MQ=255) aTCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGttct > 1:2359707/1‑67 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:44806/69‑1 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:493832/69‑1 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:1431260/69‑1 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2377504/69‑1 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:3254899/69‑1 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2875580/69‑1 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:109695/69‑1 (MQ=255) aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCACTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2681446/69‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2740007/64‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:220941/69‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:2041467/69‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:1049718/69‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:994524/69‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:1246398/69‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:1277543/69‑1 (MQ=255) catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:744356/69‑1 (MQ=255) atcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:2119599/68‑1 (MQ=255) atcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:2192660/68‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:623025/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:2414910/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1600445/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:389425/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:3354140/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1597643/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:802687/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1483979/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:3341254/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:583771/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:2833197/69‑1 (MQ=255) cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:2273450/69‑1 (MQ=255) gCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1067652/68‑1 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgtc > 1:1519527/1‑56 (MQ=38) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCgg > 1:778881/1‑68 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2252758/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1089744/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1491581/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1608054/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1704944/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1741820/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:491290/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2603456/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:393056/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2785853/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2665815/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:3407129/1‑69 (MQ=255) tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:3407133/1‑69 (MQ=255) gAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCa > 1:161942/1‑69 (MQ=255) cTGCGCGGTGGCAATCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:1688747/69‑1 (MQ=255) cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:3228117/69‑1 (MQ=255) cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:1356539/69‑1 (MQ=255) cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:260397/69‑1 (MQ=255) cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:203053/69‑1 (MQ=255) cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:2200783/69‑1 (MQ=255) cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:475440/69‑1 (MQ=255) ggCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:2505453/60‑1 (MQ=255) gCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCa > 1:2764068/1‑36 (MQ=255) gCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCa > 1:3097974/1‑36 (MQ=255) gCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCa > 1:1483406/1‑36 (MQ=255) | ACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCC > minE/1679076‑1679184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |