| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,679,847 | G→A | 29.9% | R55R (CGC→CGT) | pbpC ← | fused transglycosylase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,679,847 | 0 | G | A | 29.9% | 47.5 / 19.8 | 50 | R55R (CGC→CGT) | pbpC | fused transglycosylase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base G (9/26); new base A (12/3); total (21/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.50e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
ACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGG > minE/1679787‑1679909 | aCGTGGAGAAACATCTTCGATTGTTACTGGATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCagag < 1:1013791/69‑1 (MQ=255)aCGTGGAGAAACATCTTCGATTGTTACTGGATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCagag < 1:1183915/69‑1 (MQ=255)aCGTGGAGAAACATCTTCGATTGTTACTGGATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCagag < 1:585360/69‑1 (MQ=255) aCCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGAc < 1:1314839/68‑1 (MQ=255) aCCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGAc < 1:910781/68‑1 (MQ=255) aCCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGAc < 1:69460/68‑1 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCAt > 1:1169010/3‑54 (MQ=38) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCAt > 1:2762369/3‑54 (MQ=38) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGccaccacca > 1:65101/3‑69 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGccaccacca > 1:1790444/3‑69 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGccaccacca > 1:1876962/3‑69 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGccaccacca > 1:2915755/3‑69 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGccaccacca > 1:945285/3‑69 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGccaccacca > 1:2467055/3‑69 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGcc > 1:1343055/3‑62 (MQ=39) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGCCaccacc > 1:3148372/3‑68 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTGCGCCaccacc > 1:2615319/3‑68 (MQ=255) ctggATAACGCCAGATGCCGTCAGCATCGGCGAAACGCCAGAGTGGCGTACCATCCTACGccaccacca > 1:2077950/3‑69 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTg > 1:2660592/1‑63 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCggg > 1:391637/1‑67 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGa > 1:1363845/1‑68 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGa > 1:3307787/1‑68 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGa > 1:3433260/1‑68 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGa > 1:1915854/1‑68 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGa > 1:751581/1‑68 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGa > 1:893258/1‑68 (MQ=255) aGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGa > 1:2365515/1‑68 (MQ=255) ccGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt < 1:2209589/69‑1 (MQ=255) ccGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt < 1:2663726/69‑1 (MQ=255) ccGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt < 1:2752940/69‑1 (MQ=255) ccGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt < 1:1409724/69‑1 (MQ=255) ccGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt < 1:1534050/69‑1 (MQ=255) ccGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt < 1:3159159/69‑1 (MQ=255) ccGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt < 1:2431300/69‑1 (MQ=255) gTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:972870/68‑1 (MQ=255) gTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:367347/68‑1 (MQ=255) gTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:3224782/68‑1 (MQ=255) gTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:2672010/68‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:3106685/62‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:571439/62‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:1913514/62‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:2612499/62‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:86819/62‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:2482072/62‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:1329388/62‑1 (MQ=255) aTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:2297204/62‑1 (MQ=255) tCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt < 1:1883650/61‑1 (MQ=255) ggCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg < 1:1751540/69‑1 (MQ=255) ggCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg < 1:685693/69‑1 (MQ=255) ggCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg < 1:1340985/69‑1 (MQ=255) | ACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGG > minE/1679787‑1679909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |