| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,680,429 | A→G | 36.7% | D1515D (GAT→GAC) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,680,429 | 0 | A | G | 36.7% | 45.2 / 48.3 | 79 | D1515D (GAT→GAC) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (31/19); new base G (8/21); total (39/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCG > minE/1680363‑1680493 | ttttACCTGCAACCATACCAGCACCAGATCTCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATc < 1:353064/69‑1 (MQ=255)ttttACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATc < 1:157423/69‑1 (MQ=255)ttttACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATc < 1:225942/69‑1 (MQ=255)ttttACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATc < 1:2626522/69‑1 (MQ=255) acacCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAatat > 1:2230593/1‑69 (MQ=255) acacCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAatat > 1:1360146/1‑69 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGaa > 1:869739/1‑57 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAatat > 1:2361200/1‑60 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGa > 1:2647119/1‑69 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGa > 1:2080480/1‑69 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGa > 1:368050/1‑69 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGa > 1:1916803/1‑69 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGa > 1:2361780/1‑69 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGa > 1:615176/1‑69 (MQ=255) ccagATCGCCGCTACGTAACGAGTCCAGCGACTTGCTCTTACCATCAGTACCAAGAATATGAAGCTCGa > 1:1291055/1‑69 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:2992885/1‑44 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:961182/1‑44 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:228796/1‑44 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:232692/1‑44 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:1067007/1‑44 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:786924/1‑44 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:1332502/1‑44 (MQ=255) aCGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGaa > 1:85235/1‑44 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:3257791/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2977438/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:3168181/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2354138/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:3006385/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2634297/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2299635/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:209842/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1168551/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1402823/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:761137/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1475999/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:701273/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:630421/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1544247/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1606064/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:431392/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2041503/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2191917/69‑1 (MQ=255) aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCACCACATTGCTc < 1:2069956/69‑1 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTc > 1:1783113/1‑63 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCgg > 1:3340585/1‑68 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCgg > 1:193906/1‑68 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCgg > 1:2632810/1‑68 (MQ=255) cAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTaa < 1:2477789/69‑1 (MQ=255) cGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1829940/58‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1119687/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1493398/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:3416577/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:2419497/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1417996/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:2445363/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:3254433/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1305825/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:266390/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:2978992/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:2801221/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:126743/69‑1 (MQ=255) tGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1487190/68‑1 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAgg > 1:786730/1‑58 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:861807/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:541742/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:2401994/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:518988/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:2021227/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:2580015/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:3134919/1‑69 (MQ=255) aCCATCAGTACCAATAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAgg < 1:3285114/57‑1 (MQ=255) aCCATCAGTACCAAGAATATGCCGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:1790379/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGc > 1:2876206/1‑48 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:478502/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:2842619/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:3429185/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:2628371/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:2956425/1‑68 (MQ=255) aCCATCAGTACCAAGAAAATGACGCTCGATTTGCAGCACATAGTTCGCCGGTAAAGGtgcggattgcg > 1:1343807/1‑68 (MQ=255) | TTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCG > minE/1680363‑1680493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |