| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 130,756 | C→G | 53.3% | R312R (CGC→CGG) | yadE → | predicted polysaccharide deacetylase lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 130,756 | 0 | C | G | 53.3% | 11.5 / 86.9 | 75 | R312R (CGC→CGG) | yadE | predicted polysaccharide deacetylase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (22/13); new base G (19/21); total (41/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCG > minE/130690‑130820 | cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:995058/1‑69 (MQ=255)cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:2148885/1‑69 (MQ=255)cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:2405231/1‑69 (MQ=255)cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:2869332/1‑69 (MQ=255)cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:1257080/1‑69 (MQ=255)cGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCg > 1:635098/1‑69 (MQ=255)cGAAATTCGCGATGTATTTGATTT‑CAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGa > 1:3050963/1‑69 (MQ=255) aTTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATGGGGTAGATGGTTATCGCCGAccc < 1:2979932/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1259238/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1348653/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1433423/69‑1 (MQ=255) ttCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1573818/69‑1 (MQ=255) gATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa < 1:1915540/64‑1 (MQ=255) tttGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc > 1:2620451/1‑69 (MQ=255) aTTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGt < 1:3066171/68‑1 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTg > 1:2452447/1‑68 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1761935/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:309454/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1940560/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:197627/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:2388706/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3279268/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3256514/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1049846/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1342739/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1466739/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1300204/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1079912/1‑69 (MQ=255) aTTTTTTGCATAGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3074273/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGa > 1:1123344/1‑42 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:550340/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:473200/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:1659862/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:2822523/1‑69 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3413015/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:108989/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2783278/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:714824/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3055156/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1328291/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3001774/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2930983/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2920247/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:93002/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1739439/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1876398/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2244063/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2677249/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:229415/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1049094/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:216183/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1385321/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2506743/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2676678/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1513064/48‑1 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:46366/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:1495543/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:2067525/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:3402962/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:2162444/4‑69 (MQ=255) tAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGtt < 1:1335104/69‑1 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:149634/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:3363384/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:848971/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:1215901/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2276658/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2426632/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2575882/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg < 1:2861675/58‑1 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:135888/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2781839/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg < 1:1891845/58‑1 (MQ=255) aTCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg < 1:2245514/69‑1 (MQ=255) aTCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg < 1:273883/69‑1 (MQ=255) aTCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg < 1:2696447/69‑1 (MQ=255) | CGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCG > minE/130690‑130820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |