| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,680,469 | T→C | 37.5% | N1502S (AAC→AGC) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,680,469 | 0 | T | C | 37.5% | 66.8 / 78.0 | 120 | N1502S (AAC→AGC) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (39/36); new base C (8/37); total (47/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
AACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATC > minE/1680403‑1680533 | aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2354138/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1606064/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:3168181/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1475999/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:3006385/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2977438/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:431392/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2041503/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2634297/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:630421/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2191917/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:761137/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:1168551/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2299635/69‑1 (MQ=255)aaCGAGTCCAGCGATTTGCTCTTACCGTCAGTACCCAGAATATGACGCTCAATTTGCACCACATTGCTc < 1:2069956/69‑1 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTc > 1:1783113/1‑63 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCgg > 1:193906/1‑68 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCgg > 1:2632810/1‑68 (MQ=255) tCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCgg > 1:3340585/1‑68 (MQ=255) cAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTaa < 1:2477789/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:3254433/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:2801221/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:266390/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1305825/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1119687/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:2978992/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1417996/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:2419497/69‑1 (MQ=255) ttGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1493398/69‑1 (MQ=255) tGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcg < 1:1487190/68‑1 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAgg > 1:786730/1‑58 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:2401994/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:2580015/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:861807/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:541742/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:518988/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:2021227/1‑69 (MQ=255) tACCGTCAGTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGtgcggattgcg > 1:3134919/1‑69 (MQ=255) aCCATCAGTACCAATAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAgg < 1:3285114/57‑1 (MQ=255) aCCATCAGTACCAAGAATATGCCGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:1790379/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGc > 1:2876206/1‑48 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:478502/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:2842619/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:2628371/1‑68 (MQ=255) aCCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:3429185/1‑68 (MQ=255) aCCATCAGTACCAAGAAAATGACGCTCGATTTGCAGCACATAGTTCGCCGGTAAAGGtgcggattgcg > 1:1343807/1‑68 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATaa > 1:1219209/1‑68 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:2814725/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:2517755/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:299739/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:1002770/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:2377492/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:2467901/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:1722404/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:174454/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:1392279/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:194149/1‑69 (MQ=255) cAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAc > 1:2373438/1‑69 (MQ=255) aGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAgg > 1:1233534/1‑51 (MQ=255) aGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAAcc > 1:2956466/1‑69 (MQ=255) gTACCCAGAATATGACGCTCAATTTGCAGCACATTGCTc < 1:2071604/39‑1 (MQ=37) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:321050/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:1582317/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:3150730/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:933476/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:3353364/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:1869846/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:1905501/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:1909310/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:69363/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:625569/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:2047792/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:1284607/69‑1 (MQ=255) aaGAATATGACGCTCGATTTGCAGCACATTGTCCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGc < 1:1574756/69‑1 (MQ=255) aaTATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATc > 1:928416/1‑69 (MQ=255) aaTATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATc > 1:657406/1‑69 (MQ=255) aaTATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATc > 1:2134629/1‑69 (MQ=255) tatGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATa > 1:3364948/1‑55 (MQ=255) tGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATa > 1:81239/1‑69 (MQ=255) tGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATa > 1:123617/1‑69 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:764521/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:112294/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:2627033/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:1642610/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:822842/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:221984/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:2204418/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:1281737/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:1001343/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:2523968/69‑1 (MQ=255) gCTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:942816/69‑1 (MQ=255) cTCAATTTGCAGCACATTGCTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCACACGCa < 1:3385836/68‑1 (MQ=255) cacaTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTg < 1:656060/69‑1 (MQ=255) cacaTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTg < 1:722382/69‑1 (MQ=255) cacaTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTg < 1:1230269/69‑1 (MQ=255) cacaTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTg < 1:282009/69‑1 (MQ=255) cacaTTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTg < 1:1738078/69‑1 (MQ=255) ttGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATc > 1:1206220/1‑68 (MQ=255) ttGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATc > 1:1531819/1‑68 (MQ=255) ttGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATc > 1:1694983/1‑68 (MQ=255) | AACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATC > minE/1680403‑1680533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |