| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,681,250 | G→A | 30.8% | L1242L (CTG→TTG) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,681,250 | 0 | G | A | 30.8% | 75.3 / 37.7 | 81 | L1242L (CTG→TTG) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (16/40); new base A (17/8); total (33/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01 | |||||||||||
AAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGACC > minE/1681195‑1681308 | aaaTCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCaaa > 1:228487/1‑62 (MQ=255)aaaTCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCaaa > 1:1835844/1‑62 (MQ=255)aaaTCCATCACGTAAGCCGTCAGCCAGTACTCTTCATCACCGTTTTTATCCCACAGCGCaaa > 1:2197829/1‑62 (MQ=255) ccATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 1:3267667/67‑1 (MQ=255) ccATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 1:2628813/67‑1 (MQ=255) ccATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 1:1812676/67‑1 (MQ=255) ccATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 1:1771506/67‑1 (MQ=255) aCGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 1:3274709/62‑1 (MQ=255) gTAAGCAGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCTCCGTTATCACGttc < 1:1755576/69‑2 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGttac < 1:2371071/69‑3 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:1593091/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:2965198/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:2101065/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:1999537/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:1854388/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:641786/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:3388687/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:3065867/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:3092849/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:3433881/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:1186464/69‑1 (MQ=255) tAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:2835133/69‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCTACAGCGCAAAGCCGCCGt < 1:211064/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:189862/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:447357/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:2924451/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:2215934/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:2165156/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:2971021/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:601960/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:3286757/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:3125531/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:877312/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:1223382/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:1152368/56‑1 (MQ=255) aGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt < 1:1025186/56‑1 (MQ=255) gTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 1:1589621/53‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:3237849/68‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:3267875/68‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:2842054/68‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:2693188/68‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:2541107/68‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:2320159/68‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:2194578/68‑1 (MQ=255) ccAGTACTCTTCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:1460651/68‑1 (MQ=255) cAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 1:37992/47‑1 (MQ=255) aGTACTCTTCGTCACCGTTTTTATCCCACAGCGCATAGCCGCCGtt < 1:1571284/46‑1 (MQ=255) aCTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACgg > 1:288471/1‑68 (MQ=255) ctctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTg > 1:904508/1‑57 (MQ=255) ctctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTg > 1:3423235/1‑57 (MQ=255) ctctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTg > 1:2028637/1‑57 (MQ=255) ctctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTg > 1:669497/1‑57 (MQ=255) ctctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGa < 1:587238/69‑1 (MQ=255) ctctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGa > 1:792497/1‑69 (MQ=255) ctctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGa < 1:2846224/69‑1 (MQ=255) ctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGaaa > 1:949304/1‑69 (MQ=255) ctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGaaa > 1:965312/1‑69 (MQ=255) ctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGaaa > 1:2230637/1‑69 (MQ=255) ctTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGaaa > 1:2143055/1‑69 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTAt > 1:466578/1‑41 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:594864/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:1024338/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:1120490/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:1222991/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:895233/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:1345394/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:1505883/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:748499/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:154887/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:1789489/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:2126560/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:3171664/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:581139/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:2476304/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:2964904/1‑42 (MQ=255) ttCGTCACCGTTTTTATCCCACAACGCAAAGCCGCCGTTATc > 1:30364/1‑42 (MQ=255) tttATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGa > 1:2220472/1‑68 (MQ=255) tttATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGa > 1:778659/1‑68 (MQ=255) tttATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGa > 1:2727899/1‑68 (MQ=255) tATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGAcc < 1:3054737/68‑1 (MQ=255) tATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGAcc < 1:3156669/68‑1 (MQ=255) | AAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGGGAAATGCCGATATCGACC > minE/1681195‑1681308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |