| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,682,068 | A→G | 38.1% | V969A (GTG→GCG) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,682,068 | 0 | A | G | 38.1% | 55.2 / 69.5 | 105 | V969A (GTG→GCG) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (25/40); new base G (24/16); total (49/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-02 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCAC > minE/1682004‑1682122 | gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:2286254/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:1981859/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:3175825/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:587748/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:734382/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:186881/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:2113659/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:2122726/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:785354/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:1425819/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:3325467/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:331349/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:1191081/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:447262/69‑1 (MQ=255)gCCAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:1222846/69‑1 (MQ=255)gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGAc < 1:3418734/69‑1 (MQ=255)gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGAc < 1:1162639/69‑1 (MQ=255)gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGAc < 1:1868801/69‑1 (MQ=255)gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGAc < 1:1856670/69‑1 (MQ=255)gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGAc < 1:1350059/69‑1 (MQ=255)gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGAc < 1:766335/69‑1 (MQ=255) ccAATTGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGAc < 1:217824/68‑1 (MQ=255) cAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACaa < 1:2395666/69‑1 (MQ=255) cAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACaa < 1:2988268/69‑1 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:2272582/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:31148/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:272667/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:56315/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:2455394/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:2006159/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:2851411/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:1999259/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:1799897/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:3276041/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAata > 1:1155144/3‑68 (MQ=255) ttggCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAat > 1:1905426/3‑67 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:312276/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:962471/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:3316970/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:1360707/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:3300227/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:3111897/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:3246139/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:1627865/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:740418/69‑1 (MQ=255) gtgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:717767/69‑1 (MQ=255) gtgtAACCGAGCCTTCGACCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:1906723/69‑1 (MQ=255) tgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGGCTGCTGCACGACAATATTGACGt < 1:2213315/68‑1 (MQ=255) tgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:529428/68‑1 (MQ=255) tgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:769540/68‑1 (MQ=255) tgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:2484589/68‑1 (MQ=255) tAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAatat > 1:421539/1‑60 (MQ=255) tAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTg > 1:3168738/1‑67 (MQ=255) tAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTg > 1:2107193/1‑67 (MQ=255) tAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTg > 1:1075079/1‑67 (MQ=255) tAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTg > 1:953588/1‑67 (MQ=255) tAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTg > 1:1797827/1‑67 (MQ=255) tAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTg > 1:2809154/1‑67 (MQ=255) aCCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTg < 1:492243/69‑1 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACaa > 1:1586086/1‑52 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:667579/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:1344907/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:879937/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:1456291/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:609203/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:581672/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:3059795/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:2469486/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:2476055/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:2858981/1‑64 (MQ=255) gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCGCGACAATATTGACGTGAt > 1:2555819/1‑64 (MQ=255) ccTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:1925855/58‑1 (MQ=255) ccTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac < 1:981432/69‑1 (MQ=255) ccTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac < 1:3421364/69‑1 (MQ=255) ccTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac < 1:889435/69‑1 (MQ=255) ccTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac < 1:1986259/69‑1 (MQ=255) ccTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac < 1:1959678/69‑1 (MQ=255) cTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac < 1:1474594/68‑1 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:3244854/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:1174895/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:1267509/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:1372858/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:2574950/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:2511478/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:2407671/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc > 1:1519550/1‑69 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccac > 1:3319446/1‑68 (MQ=255) ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGAAAATATTGACGt > 1:1028295/1‑47 (MQ=255) cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt > 1:1941281/1‑45 (MQ=255) cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt > 1:1889510/1‑45 (MQ=255) cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt > 1:606360/1‑45 (MQ=255) cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt > 1:2907923/1‑45 (MQ=255) cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt > 1:2964691/1‑45 (MQ=255) cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt > 1:3216376/1‑45 (MQ=255) gAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGt < 1:1215284/41‑1 (MQ=255) gCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACTGGCGGTTTACCACCACGTTTc < 1:3317094/68‑1 (MQ=255) ttACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCt < 1:2047640/69‑1 (MQ=255) gCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCAc < 1:1811356/69‑1 (MQ=255) gCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCAc < 1:1509339/69‑1 (MQ=255) gCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCAc < 1:942947/69‑1 (MQ=255) | GCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCAC > minE/1682004‑1682122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |