| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,683,045 | G→A | 62.0% | G643G (GGC→GGT) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,683,045 | 0 | G | A | 62.0% | 34.7 / 45.1 | 71 | G643G (GGC→GGT) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (23/4); new base A (5/39); total (28/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
CAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCG > minE/1682979‑1683111 | cAAGCTCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTAcc > 1:1202227/1‑69 (MQ=255)cAAGCTCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTAcc > 1:2372161/1‑69 (MQ=255)cAAGCTCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTAcc > 1:1519365/1‑69 (MQ=255)cAAGCTCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTAcc > 1:2397876/1‑69 (MQ=255)cAAGCTCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTAcc > 1:3393129/1‑69 (MQ=255) aGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTGcc < 1:444012/67‑1 (MQ=255) aGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTGcc < 1:1857362/67‑1 (MQ=255) aGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTGCCtt < 1:233829/69‑1 (MQ=255) gCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGt > 1:79924/1‑69 (MQ=255) gCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGt > 1:527124/1‑69 (MQ=255) gCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGt > 1:2142719/1‑69 (MQ=255) gCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGt > 1:868462/1‑69 (MQ=255) gCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGt > 1:875482/1‑69 (MQ=255) gCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGt > 1:3316359/1‑69 (MQ=255) gCTATAAAAGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGt > 1:1379929/1‑69 (MQ=255) aaCGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATa > 1:1188591/1‑69 (MQ=255) aaCGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATa > 1:3153878/1‑69 (MQ=255) gATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAAt > 1:357810/1‑68 (MQ=255) gATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAAt > 1:792164/1‑68 (MQ=255) gATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAAt > 1:2594032/1‑68 (MQ=255) gATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAAt > 1:2529906/1‑68 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:3266887/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:3013886/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:3031581/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:3148431/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:961808/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:3162864/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:317709/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:616086/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:897311/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:3436193/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:76790/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:735993/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:734481/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:621744/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:1237483/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2718891/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:1540530/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2682254/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2510281/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2483972/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2395349/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2428572/69‑1 (MQ=255) gcgTTACTACCTTCATCAACAATCGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:1166086/69‑1 (MQ=255) cgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:562521/68‑1 (MQ=255) cgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:889335/68‑1 (MQ=255) cgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:1820982/68‑1 (MQ=255) cgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2687100/68‑1 (MQ=255) cgTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTc < 1:2836517/68‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:2401662/69‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:199358/69‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:2059769/69‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:221487/69‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:2975905/69‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:933790/69‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:2412335/69‑1 (MQ=255) gTTACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:392885/69‑1 (MQ=255) ttACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGAt > 1:2733207/1‑63 (MQ=255) ttACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAg > 1:598412/1‑69 (MQ=255) ttACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAg > 1:235686/1‑69 (MQ=255) ttACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:2287587/68‑1 (MQ=255) ttACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:825112/68‑1 (MQ=255) ttACTACCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCGGTGCGATAATCGTAAACCGATTTCGa < 1:863648/68‑1 (MQ=255) aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTc > 1:1202298/1‑39 (MQ=255) aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTc > 1:1764303/1‑39 (MQ=255) aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAgg > 1:3023857/1‑68 (MQ=255) aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAgg > 1:2004817/1‑68 (MQ=255) aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAgg > 1:614611/1‑68 (MQ=255) aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAgg > 1:417087/1‑68 (MQ=255) aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAg > 1:3309337/1‑67 (MQ=255) tGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCg < 1:3342086/68‑1 (MQ=255) | CAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAACGATGTCAAAAGCGGCGTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCG > minE/1682979‑1683111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |