| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,683,429 | A→G | 50.0% | G515G (GGT→GGC) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,683,429 | 0 | A | G | 50.0% | ‑5.1 / 32.2 | 38 | G515G (GGT→GGC) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/17); new base G (2/17); total (4/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTT > minE/1683362‑1683480 | cTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCAc < 1:1942713/69‑1 (MQ=255)cTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCAc < 1:1398979/69‑1 (MQ=255) tCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGgtag < 1:1242996/69‑1 (MQ=255) tCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGgtag < 1:2013370/69‑1 (MQ=255) tCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGgtag < 1:1729372/69‑1 (MQ=255) tCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGgtag < 1:1408902/69‑1 (MQ=255) cGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGgtagt < 1:2949719/67‑1 (MQ=255) tGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCccac < 1:789096/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:673280/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:414778/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:791682/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2556078/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:403102/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:3122028/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2901494/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2880732/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2745676/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:1065061/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:1323820/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:1437284/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2267665/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:1514926/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2069768/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2152414/69‑1 (MQ=255) gggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCGCCATACAGGTAGTACCccacc < 1:2070866/69‑1 (MQ=255) ggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGgtag > 1:215683/1‑59 (MQ=255) ggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCccacca > 1:290144/1‑69 (MQ=255) aTTACCATTAGCAGGTGCGCCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGc > 1:2669488/1‑67 (MQ=255) aTTACCATTAGCAGGTGCGCCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGc > 1:3016613/1‑67 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:89274/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:2420504/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:1545185/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:2217765/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:2851173/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:2797041/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:2745461/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:2621814/69‑1 (MQ=255) ttACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGtt < 1:2401454/69‑1 (MQ=255) | CTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTT > minE/1683362‑1683480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |