| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,683,516 | G→A | 16.2% | R486R (CGC→CGT) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,683,516 | 0 | G | A | 16.2% | 102.4 / 8.5 | 61 | R486R (CGC→CGT) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (30/21); new base A (5/5); total (35/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01 | |||||||||||
ACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCG > minE/1683451‑1683582 | acGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATg > 1:1131661/1‑66 (MQ=255)acGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATg > 1:2673204/1‑66 (MQ=255)acGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATg > 1:1172914/1‑66 (MQ=255)acGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATg > 1:2712788/1‑66 (MQ=255)acGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATg > 1:2376981/1‑66 (MQ=255) tgCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:302640/55‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATgcg > 1:335309/1‑38 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:1024534/56‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:353002/56‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:2019522/56‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:554467/56‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:564667/56‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:68278/56‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTc < 1:239105/56‑1 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAAt > 1:1854131/1‑67 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:2030423/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:1692343/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:2014224/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:1807382/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:3267368/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:1747016/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:1444542/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:472036/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:1265058/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:682067/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:809347/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:892674/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc > 1:974456/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGACATAAAATCTTCGACGTGGAAATcc > 1:211726/1‑69 (MQ=255) aGCGGGGTTTTCTCACCGGTCAGATTCACCGCCATGCGCTCTGGCATAAAATCTTc < 1:2769336/56‑1 (MQ=255) ggggTTTTCTCACCGGTCAGATTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCcac > 1:312921/1‑69 (MQ=255) ggggTTTTCTCACCGGTCAGATTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCcac > 1:2579507/1‑69 (MQ=255) ggggTTTTCTCACCGGTCAGATTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCcac > 1:682245/1‑69 (MQ=255) ggggTTTTCTCACCGGTCAGATTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCcac > 1:1136886/1‑69 (MQ=255) ggggTTTTCTCACCGGTCAGATTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCcac > 1:1149327/1‑69 (MQ=255) ttCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCcaca > 1:3410787/1‑64 (MQ=255) ttCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGa > 1:2554210/1‑69 (MQ=255) ttCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGa > 1:2378919/1‑69 (MQ=255) ttCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGa > 1:1334253/1‑69 (MQ=255) ttCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGa > 1:1654152/1‑69 (MQ=255) cAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc < 1:1180345/49‑1 (MQ=255) cAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc < 1:2967322/49‑1 (MQ=255) cAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATcc < 1:512755/49‑1 (MQ=255) cAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATc > 1:902758/1‑69 (MQ=255) cAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATc > 1:1258687/1‑69 (MQ=255) cAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATc > 1:672441/1‑69 (MQ=255) cAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATc > 1:391191/1‑69 (MQ=255) aTTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGGTTATCGcc < 1:2614284/69‑1 (MQ=255) aTTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGGTTATCGcc < 1:539612/69‑1 (MQ=255) aTTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGGTTATCGcc < 1:1755498/69‑1 (MQ=255) aTTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGGTTATCGcc < 1:3250546/69‑1 (MQ=255) aTTCAGCGCCATACGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGGTTATCGcc < 1:2346910/69‑1 (MQ=255) tCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCg < 1:1591397/69‑1 (MQ=255) cAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCg < 1:1408709/68‑1 (MQ=255) cgcCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGtt < 1:1029146/69‑1 (MQ=255) cgcCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGtt < 1:965285/69‑1 (MQ=255) cgcCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGtt < 1:2653625/69‑1 (MQ=255) cgcCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGtt < 1:2529474/69‑1 (MQ=255) cgcCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGtt < 1:2250846/69‑1 (MQ=255) cgcCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGtt < 1:1354410/69‑1 (MQ=255) cgcCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGtt < 1:1794654/69‑1 (MQ=255) tGCGCTCTGGCATAAATTCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAgcg > 1:2873815/1‑68 (MQ=255) tGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAgcg > 1:27564/1‑68 (MQ=255) tGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAgcg > 1:2676600/1‑68 (MQ=255) tGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAgcg > 1:767240/1‑68 (MQ=255) tGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAgcg > 1:827304/1‑68 (MQ=255) tGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAgcg > 1:1133893/1‑68 (MQ=255) | ACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCG > minE/1683451‑1683582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |