Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,683,719 T→C 57.0% N419D (AAT→GAT)  yfhM ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,683,7190TC57.0% 19.0 / 47.2 65N419D (AAT→GAT) yfhMconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/13);  new base C (16/21);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATT  >  minE/1683655‑1683765
                                                                |                                              
tGACTAACGACGCTCCTTAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGc                                            >  1:1877038/1‑69 (MQ=255)
tGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGc                                            >  1:886237/1‑69 (MQ=255)
     aaCGACGCTCCTGAGTACCTGCCCATCGTGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGc                                       <  1:2459179/69‑1 (MQ=255)
     aaCGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGc                                       <  1:614646/69‑1 (MQ=255)
     aaCGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGc                                       <  1:694354/69‑1 (MQ=255)
     aaCGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGc                                       <  1:2653533/69‑1 (MQ=255)
        gacgCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCt                                      <  1:969753/67‑1 (MQ=255)
        gacgCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCt                                      <  1:606665/67‑1 (MQ=255)
        gacgCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCt                                      <  1:460696/67‑1 (MQ=255)
        gacgCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCt                                      <  1:698084/67‑1 (MQ=255)
        gacgCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCt                                      <  1:2418070/67‑1 (MQ=255)
        gacgCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCt                                      <  1:2311864/67‑1 (MQ=255)
        gacgCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCt                                      <  1:196901/67‑1 (MQ=255)
                 gAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTg                            >  1:2429865/1‑68 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:489777/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:3335237/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:3290775/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:3246325/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:3356685/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:336591/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:2928834/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:2826283/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:1865500/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:1457626/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:1348538/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:952189/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:926350/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:1665748/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:2422457/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:2380464/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:177394/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:212967/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:2038813/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:1187174/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGt                               <  1:655386/50‑1 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCa                       >  1:2728039/1‑58 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:841929/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:485662/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:556391/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:1293189/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:3164124/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:724698/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:2673069/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:2669791/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:2669137/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:935295/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:928296/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:2365343/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:1974177/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACCGTCTGCATCACGCAGCAAAcc            >  1:1851685/1‑69 (MQ=255)
                                gggTTTAATCACGTCTAACTTGATGGGTTGATCGGGCAATGCTTTACAGTCTGCATCACGCAGGAAAcc            >  1:332482/1‑69 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:2755689/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:645717/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:636895/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:420240/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:3061600/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:111165/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:2597501/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:1693937/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:1648877/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:1127240/1‑68 (MQ=255)
                                     tAATCACGTCTAACTTGATGGGTTGATTGGGCAAAGCTTTACCGTCTGCATCACGCAGCAAACCATTg        >  1:1622591/1‑68 (MQ=255)
                                        tCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAgg     <  1:581583/68‑1 (MQ=255)
                                          aCGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCa                       <  1:2648740/48‑1 (MQ=255)
                                          aCGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCCCGCAGCAAACCATTGAGGAtt  >  1:874393/1‑69 (MQ=255)
                                                                |                                              
TGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATT  >  minE/1683655‑1683765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: